对溶组织内阿米巴诱导保护性免疫——一项实验研究。

Elicitation of protective immunity to Entamoeba histolytica--an experimental study.

作者信息

Nain P C, Vinayak V K

出版信息

Immunol Cell Biol. 1987 Jun;65 ( Pt 3):217-22. doi: 10.1038/icb.1987.24.

DOI:10.1038/icb.1987.24

Abstract

Immunization of hamsters with plasma membrane (PM) antigens of virulent subline of axenic Entamoeba histolytica (NIH: 200 V) entrapped in multilamellar phosphatidyl choline liposomes conferred 100% protection to a subsequent intrahepatic amoebic challenge. In contrast, vaccination of hamsters with live amoebic trophozoites injected intradermally failed to protect any of the animals. The protected animals had significantly high levels of anti-PM anti-amoebic antibodies, cellular sensitization and macrophage-mediated antibody-dependent cytotoxicity against amoebic trophozoites. However, none of the intradermally immunized animals had anti-PM anti-amoebic antibodies at the time of challenge. Such animals also had significantly low macrophage-mediated antibody-dependent cellular cytotoxicity. The data indicate the potential prophylactic use of PM antigens against hepatic amoebic infection.

摘要

用包裹在多层磷脂酰胆碱脂质体中的无菌溶组织内阿米巴强毒株（NIH: 200 V）的质膜（PM）抗原免疫仓鼠，可使其对随后的肝内阿米巴攻击产生100%的保护作用。相比之下，给仓鼠皮内注射活的阿米巴滋养体进行疫苗接种，未能保护任何一只动物。受到保护的动物具有显著高水平的抗PM抗阿米巴抗体、细胞致敏作用以及巨噬细胞介导的针对阿米巴滋养体的抗体依赖性细胞毒性。然而，在攻击时，皮内免疫的动物均没有抗PM抗阿米巴抗体。这类动物的巨噬细胞介导的抗体依赖性细胞毒性也显著较低。数据表明PM抗原在预防肝阿米巴感染方面具有潜在用途。