伊朗火鸡中禽偏肺病毒的检测与分型
Detection and subtyping avian metapneumovirus from turkeys in Iran.
作者信息
Mayahi Mansour, Momtaz Hassan, Jafari Ramezan Ali, Zamani Pejman
机构信息
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Veterinary Medicine, Veterinary Microbiology Section, Islamic Azad University of Shahrekord, Shahrekord, Iran.
出版信息
Vet Res Forum. 2017 Spring;8(2):105-108. Epub 2017 Jun 15.
Avian metapneumovirus (aMPV) causes diseases like rhinotracheitis in turkeys, swollen head syndrome in chickens and avian rhinotracheitis in other birds. Causing respiratory problems, aMPV adversely affects production and inflicts immense economic losses and mortalities, especially in turkey flocks. In recent years, several serological and molecular studies have been conducted on this virus, especially in poultry in Asia and Iran. The purpose of the present study was detecting and subtyping aMPV by reverse transcriptase polymerase chain reaction (RT-PCR) from non-vaccinated, commercial turkey flocks in Iran for the first time. Sixty three meat-type unvaccinated turkey flocks from several provinces of Iran were sampled in major turkey abattoirs. Samples were tested by RT-PCR for detecting and subtyping aMPV. The results showed that 26 samples from three flocks (4.10%) were positive for viral RNA and all of the viruses were found to be subtype B of aMPV. As a result, vaccination especially against subtype B of aMPV should be considered in turkey flocks in Iran to control aMPV infections.
禽偏肺病毒（aMPV）可引发多种疾病，如导致火鸡患鼻气管炎、鸡患肿头综合征以及其他禽类患禽鼻气管炎。aMPV引发呼吸道问题，对生产造成不利影响，带来巨大经济损失和死亡率，尤其在火鸡群中。近年来，针对该病毒开展了多项血清学和分子研究，特别是在亚洲和伊朗的家禽中。本研究的目的是首次通过逆转录聚合酶链反应（RT-PCR）对伊朗未接种疫苗的商业火鸡群中的aMPV进行检测和分型。在伊朗几个省份的主要火鸡屠宰场，采集了63个未接种疫苗的肉用火鸡群的样本。通过RT-PCR对样本进行检测，以检测和分型aMPV。结果显示，来自三个鸡群的26个样本（4.10%）病毒RNA呈阳性，且所有病毒均为aMPV B亚型。因此，为控制aMPV感染，伊朗的火鸡群应考虑接种疫苗，尤其是针对aMPV B亚型的疫苗。
Zotero 插件