Lestari Silvia W, Miati Dessy Noor, Seoharso P, Sugiyanto R, Pujianto Dwi A
a Department of Medical Biology, Faculty of Medicine , Universitas Indonesia , Jakarta , Indonesia.
b Master Program for Biomedical Sciences , Faculty of Medicine, Universitas Indonesi , Jakara , Indonesia.
Syst Biol Reprod Med. 2017 Oct;63(5):294-302. doi: 10.1080/19396368.2017.1348565. Epub 2017 Aug 8.
Asthenozoospermia, which is characterized by reduced motility, is one of the etiologies of male infertility. Its biochemical and functional consequences include altered ATPase activity. This study investigated the activities of Na, K-ATPase and Ca-ATPase and the expression of Na, K-ATPase α4 and PMCA4 isoforms in human sperm of asthenozoospermic infertile men. Nineteen samples from asthenozoospermic infertile couples were examined in this study. Computerized-assisted semen analysis (CASA) was performed, and the enzyme activity was measured based on the ability of ATPase to release organic phosphate from ATP as a substrate. The Na, K-ATPase α4 and PMCA4 isoform expression levels were measured by western immunoblotting, whereas the protein distribution was examined by immunocytochemistry. This showed that the Na, K-ATPase activity and the Na, K-ATPase α4 isoform expression were lower in the asthenozoospermia group than in the normozoospermia group (8.688±1.161 versus 13.851±1.884 µmol Pi/mg protein/h, respectively; p>0.05). In contrast, the Ca-ATPase activity was significantly higher in the asthenozoospermia group than in the normozoospermia group (11.154±1.186 versus 2.725±0.545 µmol Pi/mg protein/h, respectively; p<0.05). In comparison, PMCA4 expression in the asthenozoospermia group was lower than in the normozoospermia group (p>0.05). The altered ATPase activity and isoform expression in asthenozoospermia may impair sperm structure and function.
弱精子症以精子活力降低为特征，是男性不育的病因之一。其生化和功能后果包括ATP酶活性改变。本研究调查了弱精子症不育男性精子中钠钾ATP酶和钙ATP酶的活性以及钠钾ATP酶α4和质膜钙ATP酶4亚型的表达。本研究检测了19例弱精子症不育夫妇的样本。进行了计算机辅助精液分析（CASA），并根据ATP酶将ATP作为底物释放有机磷酸盐的能力来测量酶活性。通过蛋白质免疫印迹法测量钠钾ATP酶α4和质膜钙ATP酶4亚型的表达水平，而通过免疫细胞化学检查蛋白质分布。结果显示，弱精子症组的钠钾ATP酶活性和钠钾ATP酶α4亚型表达低于正常精子症组（分别为8.688±1.161与13.851±1.884 μmol Pi/mg蛋白质/小时；p>0.05）。相反，弱精子症组的钙ATP酶活性显著高于正常精子症组（分别为11.154±1.186与2.725±0.545 μmol Pi/mg蛋白质/小时；p<0.05）。相比之下，弱精子症组的质膜钙ATP酶4表达低于正常精子症组（p>0.05）。弱精子症中ATP酶活性和亚型表达的改变可能损害精子结构和功能。
