病毒的原子冷冻电镜结构。
Atomic cryo-EM structures of viruses.
机构信息
Department of Biological Sciences, Immunology and Infectious Disease, Purdue University, 240 S. Martin Jischke Drive, West Lafayette, IN 47907, USA; Department of Chemistry, Immunology and Infectious Disease, Purdue University, 240 S. Martin Jischke Drive, West Lafayette, IN 47907, USA; Markey Center for Structural Biology, Immunology and Infectious Disease, Purdue University, 240 S. Martin Jischke Drive, West Lafayette, IN 47907, USA; Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, 240 S. Martin Jischke Drive, West Lafayette, IN 47907, USA.
Department of Molecular Biosciences, University of Kansas, 1200 Sunnyside Avenue, Lawrence, KS 66045, USA.
出版信息
Curr Opin Struct Biol. 2017 Oct;46:122-129. doi: 10.1016/j.sbi.2017.07.002. Epub 2017 Aug 5.
During the development of single particle cryo-EM in past five decades, icosahedral viruses have led the resolution progress owing to their large mass and high symmetry. Many technical advances in cryo-EM were first established with viruses. Since reaching ∼4Å resolution in 2008, it has become a relatively routine task to solve the atomic structure of isolated viruses. The future of structural virology will be increasingly focused on remaining challenges including solving structures of jumbo viruses, intermediate functional states during assembly, maturation, and infection, and in situ structures. Recent demonstrations of near-atomic resolution structure with electron tomography and sub-tomogram averaging opens a new direction for high resolution studies of pleomorphic viruses and the pleomorphic states of icosahedral viruses that have defied past efforts using the single particle cryo-EM approach.
在过去五十年的单颗粒冷冻电镜发展过程中，由于其较大的质量和高对称性，二十面体病毒引领了分辨率的进展。许多冷冻电镜技术的进步首先是在病毒上建立的。自 2008 年达到约 4Å分辨率以来，解决分离病毒的原子结构已成为一项相对常规的任务。结构病毒学的未来将越来越关注剩余的挑战，包括解决巨型病毒的结构、组装、成熟和感染过程中的中间功能状态，以及原位结构。最近的电子断层扫描和亚断层平均技术接近原子分辨率结构的演示为多形病毒和使用单颗粒冷冻电镜方法一直难以解决的二十面体病毒的多形状态的高分辨率研究开辟了一个新的方向。
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