Gaasbeek Janzen J W, Gebhardt R, ten Voorde G H, Lamers W H, Charles R, Moorman A F
J Histochem Cytochem. 1987 Jan;35(1):49-54. doi: 10.1177/35.1.2878950.
Two days before birth, immunohistochemical detection of glutamine synthetase already reveals a heterogeneous distribution pattern related to the vascular architecture of the liver. Only a small number of hepatocytes in the vicinity of the efferent venules show relatively high staining intensity. Before that age, only megakaryocytes show intense staining, while liver parenchyma is only faintly stained. The developmental profile of glutamine synthetase activity shows two periods of increasing enzyme activity: one in the perinatal period and one in the second and third postnatal week. Both periods are correlated with high levels of circulating corticosteroid hormones. Although the relative number of intensely stained hepatocytes increases during the first rise in enzyme activity, the second rise is correlated with a decreasing number of glutamine synthetase-positive hepatocytes which, however, show a considerable increase in staining intensity. Carbamoylphosphate synthetase shows a homogeneous distribution pattern in the perinatal period. Conditions that lead during development to a relatively high level of glutamine synthetase expression in the pericentral compartment apparently originate before the appearance of conditions that lead to a relatively high level of carbamoylphosphate synthetase gene expression in the periportal compartment. Our results indicate that downstream localization of glutamine synthetase in liver acinus is essential from the perinatal period onwards, whereas reciprocal distribution of glutamine synthetase and carbamoylphosphate synthetase gene expression (that is found in adult rat liver) is not.
出生前两天，谷氨酰胺合成酶的免疫组织化学检测已显示出与肝脏血管结构相关的异质分布模式。仅在输出小静脉附近的少数肝细胞显示出相对较高的染色强度。在该年龄之前，只有巨核细胞显示出强烈染色，而肝实质仅轻微染色。谷氨酰胺合成酶活性的发育概况显示出酶活性增加的两个时期：一个在围产期，另一个在出生后第二和第三周。这两个时期都与循环皮质类固醇激素的高水平相关。尽管在酶活性首次升高期间，强烈染色的肝细胞相对数量增加，但第二次升高与谷氨酰胺合成酶阳性肝细胞数量减少相关，然而，这些肝细胞的染色强度有相当大的增加。氨甲酰磷酸合成酶在围产期显示出均匀的分布模式。在发育过程中导致中央周围区谷氨酰胺合成酶表达水平相对较高的条件显然在导致门周区氨甲酰磷酸合成酶基因表达水平相对较高的条件出现之前就已产生。我们的结果表明，从围产期开始，谷氨酰胺合成酶在肝腺泡中的下游定位是必不可少的，而谷氨酰胺合成酶和氨甲酰磷酸合成酶基因表达的相互分布（在成年大鼠肝脏中发现）则不是。
