Mousavi Monirehalsadat, Thomasson Björn, Li Meng, Kraft Marco, Würth Christian, Resch-Genger Ute, Andersson-Engels Stefan
Department of Physics, Lund University, Lund, Sweden.
Phys Chem Chem Phys. 2017 Aug 23;19(33):22016-22022. doi: 10.1039/c7cp03785f.
The quantum yield is a critically important parameter in the development of lanthanide-based upconverting nanoparticles (UCNPs) for use as novel contrast agents in biological imaging and optical reporters in assays. The present work focuses on the influence of the beam profile in measuring the quantum yield (ϕ) of nonscattering dispersions of nonlinear upconverting probes, by establishing a relation between ϕ and excitation light power density from a rate equation analysis. A resulting 60% correction in the measured ϕ due to the beam profile utilized for excitation underlines the significance of the beam profile in such measurements, and its impact when comparing results from different setups and groups across the world.
量子产率是镧系上转换纳米粒子（UCNPs）开发中的一个至关重要的参数，UCNPs用作生物成像中的新型造影剂和分析中的光学报告分子。本工作通过速率方程分析建立量子产率（ϕ）与激发光功率密度之间的关系，重点研究光束轮廓对非线性上转换探针非散射分散体量子产率（ϕ）测量的影响。由于用于激发的光束轮廓，测量得到的ϕ产生了60%的校正，这突出了光束轮廓在此类测量中的重要性，以及在比较世界各地不同装置和研究小组的结果时它所产生的影响。
