Hamedi Somayeh, Shomali Tahoora, Haghighat Aliakbar
a Department of Basic Sciences, Faculty of Veterinary Medicine, Karaj Branch , Islamic Azad University , Alborz , Iran.
b Division of Pharmacology and Toxicology, Department of Basic Sciences, School of Veterinary Medicine , Shiraz University , Shiraz , Iran.
J Diet Suppl. 2018 May 4;15(3):311-317. doi: 10.1080/19390211.2017.1345030. Epub 2017 Aug 9.
This study investigates the protective effect of Rosa damascena essential oil on diabetes-induced testicular damage in rats. Thirty-six male Wistar rats were randomly divided into 6 equal groups: Group I: negative control (no treatment); Group II: positive control (diabetic by alloxan injection); Groups III-VI that rendered diabetic and received, respectively, 50, 100, 200, and 400 µg/kg/day rose oil, orally for 28 days. Rose oil did not significantly change body weight and blood glucose level as compared to positive control. Serum testosterone level of rose oil-treated rats remained statistically the same with both negative and positive control groups (Groups I and II). Rats treated with rose oil especially at 2 higher dosages (Groups V and VI) had higher sperm count and increased diameters of seminiferous tubules as compared to Group II. Rose oil even at the lowest dosage significantly increased cell count of spermatogonia, primary spermatocytes, Sertoli cells, and Leydig cells, with better outcomes for higher dosages. It appears that short-term repeated dose administration of rose oil can dose-dependently improve structural deteriorations of testes and epididymal sperm count in diabetic rats.
本研究调查了大马士革玫瑰精油对糖尿病诱导的大鼠睾丸损伤的保护作用。36只雄性Wistar大鼠被随机分为6个相等的组：第一组：阴性对照组（未治疗）；第二组：阳性对照组（通过注射四氧嘧啶诱导糖尿病）；第三至六组诱导糖尿病并分别接受50、100、200和400μg/kg/天的玫瑰精油，口服28天。与阳性对照组相比，玫瑰精油对体重和血糖水平无显著影响。玫瑰精油处理组大鼠的血清睾酮水平在统计学上与阴性和阳性对照组（第一组和第二组）相同。与第二组相比，用玫瑰精油处理的大鼠，尤其是高剂量组（第五组和第六组），精子数量更多，生精小管直径增大。即使是最低剂量的玫瑰精油也能显著增加精原细胞、初级精母细胞、支持细胞和间质细胞的数量，高剂量组效果更好。看来短期重复给药玫瑰精油可剂量依赖性地改善糖尿病大鼠睾丸的结构恶化和附睾精子数量。
