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克氏锥虫前鞭毛体中的有包膜和无包膜病毒样颗粒。

Enveloped and non-enveloped viral-like particles in Trypanosoma cruzi epimastigotes.

作者信息

Fernández-Presas Ana María, Padilla-Noriega Luis, Becker Ingeborg, Robert Lilia, Jiménez José Agustín, Solano Sandra, Delgado Jose, Tato Patricia, Molinari José Luis

机构信息

Universidad Nacional Autónoma de México, Facultad de Medicina, Departamento de Microbiología y Parasitología, Mexico City, Mexico.

Universidad Nacional Autónoma de México, Instituto de Investigaciones Biomédicas, Mexico City, Mexico.

出版信息

Rev Inst Med Trop Sao Paulo. 2017 Aug 7;59:e46. doi: 10.1590/S1678-9946201759046.

DOI:10.1590/S1678-9946201759046
PMID:28793017
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5553942/
Abstract

Electron microscopy is routinely used to identify viral infections in protozoan parasites. These viruses have been described as non-enveloped and icosahedral structures with a diameter of 30-60 nm. Most of them are classified within the non-segmented dsRNA Totiviridae family. We observed virus-like particles (VLPs) through transmission electron microscopy in the cytoplasm of Trypanosoma cruzi epimastigotes grown in cultures. Clusters of electrodense enveloped VLPs having a diameter of 48 nm were also observed. These clusters appear to have been released from distended Golgi cisternae. Furthermore, a paracrystalline array of electrodense, non-enveloped VLPs (with a diameter of 32 nm) were found in distended Golgi cisternae or as smaller clusters at a distance from the RE or Golgi. We cannot rule out that the 48 nm enveloped VLPs belong to the ssRNA Flaviviridae family because they are within its size range. The localization of enveloped VLPs is consistent with the replication strategy of these viruses that transit through the Golgi to be released at the cell surface. Due to the size and shape of the 32 nm non-enveloped VLPs, we propose that they belong to the dsRNA Totiviridae family. This is the first description of cytoplasmic enveloped and non-enveloped VLPs in T. cruzi epimastigotes.

摘要

电子显微镜常用于鉴定原生动物寄生虫中的病毒感染。这些病毒被描述为直径30 - 60纳米的无包膜二十面体结构。其中大多数被归类于非分段双链RNA的全病毒科。我们通过透射电子显微镜在培养的克氏锥虫上鞭毛体的细胞质中观察到了病毒样颗粒（VLP）。还观察到直径为48纳米的电子致密包膜VLP簇。这些簇似乎是从扩张的高尔基体池中释放出来的。此外，在扩张的高尔基体池中或在远离内质网或高尔基体的较小簇中发现了电子致密的无包膜VLP（直径32纳米）的准晶体阵列。我们不能排除48纳米包膜VLP属于单链RNA黄病毒科，因为它们在其大小范围内。包膜VLP的定位与这些通过高尔基体转运并在细胞表面释放的病毒的复制策略一致。由于32纳米无包膜VLP的大小和形状，我们认为它们属于双链RNA全病毒科。这是首次对克氏锥虫上鞭毛体中的细胞质包膜和无包膜VLP进行描述。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24e5/5553942/7d37ac6e0daa/1678-9946-rimtsp-S1678-9946201759046-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24e5/5553942/6bc04eda1a24/1678-9946-rimtsp-S1678-9946201759046-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24e5/5553942/ef089fba5411/1678-9946-rimtsp-S1678-9946201759046-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24e5/5553942/bfe2f5979a7f/1678-9946-rimtsp-S1678-9946201759046-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24e5/5553942/cb335c43f01b/1678-9946-rimtsp-S1678-9946201759046-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24e5/5553942/7d37ac6e0daa/1678-9946-rimtsp-S1678-9946201759046-gf05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24e5/5553942/6bc04eda1a24/1678-9946-rimtsp-S1678-9946201759046-gf01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24e5/5553942/ef089fba5411/1678-9946-rimtsp-S1678-9946201759046-gf02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24e5/5553942/bfe2f5979a7f/1678-9946-rimtsp-S1678-9946201759046-gf03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24e5/5553942/cb335c43f01b/1678-9946-rimtsp-S1678-9946201759046-gf04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/24e5/5553942/7d37ac6e0daa/1678-9946-rimtsp-S1678-9946201759046-gf05.jpg

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