Schiffer Doris, Tegl Gregor, Vielnascher Robert, Weber Hansjoerg, Schoeftner Rainer, Wiesbauer Herfried, Sigl Eva, Heinzle Andrea, Guebitz Georg M
ACIB-Austrian Centre of Industrial Biotechnology, Konrad-Lorenz Strasse 203630 Tuln, Graz 8010, Austria.
Department of Environmental Biotechnology, University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Strasse 20, 3430 Tulln an der Donau, Vienna 1180, Austria.
Materials (Basel). 2015 Sep 25;8(10):6633-6639. doi: 10.3390/ma8105329.
There is a strong need for simple and fast methods for wound infection determination. Myeloperoxidase, an immune system-derived enzyme was found to be a suitable biomarker for wound infection. Hence, alkoxysilane-derivatized Fast Blue RR was immobilized via simple hydrolytic polymerization. The resulting enzyme-responsive siloxane layers were incubated with myeloperoxidase, wound fluid or hemoglobin. The reaction was monitored via HPLC measurements and the color development quantified spectrophotometrically. Myeloperoxidase was indeed able to oxidize immobilized Fast Blue RR leading to a blue colored product. No conversion was detected in non-infected wound fluids. The visible color changes of these novel materials towards blue enable an easy distinction between infected and non-infected wound fluids.
迫切需要简单快速的伤口感染检测方法。髓过氧化物酶是一种源自免疫系统的酶，被发现是伤口感染的合适生物标志物。因此，通过简单的水解聚合固定化烷氧基硅烷衍生的固蓝RR。将所得的酶响应性硅氧烷层与髓过氧化物酶、伤口渗出液或血红蛋白一起孵育。通过高效液相色谱测量监测反应，并通过分光光度法定量显色。髓过氧化物酶确实能够氧化固定化的固蓝RR，产生蓝色产物。在未感染的伤口渗出液中未检测到转化。这些新型材料向蓝色的可见颜色变化使得能够轻松区分感染和未感染的伤口渗出液。
