Jiang Shiau-Han, Cheng Yung-Yi, Huo Teh-Ia, Tsai Tung-Hu
Institute of Pharmacology, National Yang-Ming University , Taipei, Taiwan.
Institute of Traditional Medicine, National Yang-Ming University , Taipei, Taiwan.
J Agric Food Chem. 2017 Sep 6;65(35):7797-7804. doi: 10.1021/acs.jafc.7b02685. Epub 2017 Aug 21.
Rhodamine dyes have been banned as food additives due to their potential tumorigenicity. Rhodamine 110 is illegal as a food additive, although its pharmacokinetics have not been characterized, and no accurate bioanalytical methods are available to quantify rhodamine 110. The aim of this study was to develop and validate a fast, stable, and sensitive method to quantify rhodamine 110 using high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) to assess its pharmacokinetics and organ distribution in awake rats. Rhodamine 110 exhibited linear pharmacokinetics and slow elimination after oral administration. Furthermore, its oral bioavailability was approximately 34-35%. The distribution in the liver and kidney suggests that these organs are primarily responsible for rhodamine 110 metabolism and elimination. Our investigation describes the pharmacokinetics and a quantification method for rhodamine 110, improving our understanding of the food safety of rhodamine dyes.
由于罗丹明染料具有潜在的致瘤性，已被禁止用作食品添加剂。罗丹明110作为食品添加剂是非法的，尽管其药代动力学尚未得到表征，并且没有准确的生物分析方法可用于定量罗丹明110。本研究的目的是开发并验证一种快速、稳定且灵敏的方法，使用高效液相色谱-串联质谱法（HPLC-MS/MS）定量罗丹明110，以评估其在清醒大鼠体内的药代动力学和器官分布。口服给药后，罗丹明110呈现线性药代动力学且消除缓慢。此外，其口服生物利用度约为34%-35%。在肝脏和肾脏中的分布表明，这些器官主要负责罗丹明110的代谢和消除。我们的研究描述了罗丹明110的药代动力学和定量方法，增进了我们对罗丹明染料食品安全的理解。
