Crislip G Ryan, O'Connor Paul M, Wei Qingqing, Sullivan Jennifer C
Department of Physiology, Augusta University, Augusta, Georgia; and.
Department of Cellular Biology and Anatomy, Augusta University, Augusta, Georgia.
Am J Physiol Renal Physiol. 2017 Nov 1;313(5):F1097-F1105. doi: 10.1152/ajprenal.00261.2017. Epub 2017 Aug 9.
Recent evidence suggests that a greater density of pericytes in renal cadaveric allografts is associated with better recovery following transplant. The physiological mechanism(s) through which pericyte density may be beneficial is not well understood. The goal of this study was to test the hypothesis that lower medullary pericyte density is associated with greater renal injury following ischemia reperfusion (IR) in a rat model, providing a basis for future studies to better understand pericytes in a pathological environment. To test our hypothesis, we determined the association between medullary pericyte density and renal injury in spontaneously hypertensive rats (SHR) following 45 min of warm bilateral IR. We found that there was a significant negative relationship between pericyte density and plasma creatinine (slope = -0.03, = 0.02) and blood urea nitrogen (slope = -0.5, = 0.01) in female but not male SHR. Pericyte density was negatively associated with medullary peritubular capillary (PT) congestion in both sexes following IR (male: slope = -0.04, = 0.009; female: slope = -0.03, = 0.0001). To further test this relationship, we used a previously reported method to reduce pericyte density in SHR. Medullary erythrocyte congestion in vasa recta (VR) and PT significantly increased following IR in both sexes when pericyte density was pharmacologically decreased (VR: = 0.03; PT: = 0.03). Our data support the hypothesis that pericyte density is negatively associated with the development of IR injury in SHR, which may be mediated by erythrocyte congestion in the medullary vasculature.
近期证据表明，肾移植尸体供肾中周细胞密度更高与移植后更好的恢复相关。周细胞密度可能有益的生理机制尚不清楚。本研究的目的是检验以下假设：在大鼠模型中，肾髓质周细胞密度较低与缺血再灌注（IR）后更严重的肾损伤相关，为未来在病理环境中更好地理解周细胞的研究提供基础。为了验证我们的假设，我们测定了自发性高血压大鼠（SHR）在45分钟双侧温IR后髓质周细胞密度与肾损伤之间的关联。我们发现，雌性而非雄性SHR中，周细胞密度与血浆肌酐（斜率=-0.03，P=0.02）和血尿素氮（斜率=-0.5，P=0.01）之间存在显著负相关。IR后，两性的周细胞密度均与髓质肾小管周围毛细血管（PT）充血呈负相关（雄性：斜率=-0.04，P=0.009；雌性：斜率=-0.03，P=0.0001）。为了进一步检验这种关系，我们使用先前报道的方法降低SHR中的周细胞密度。当通过药物降低周细胞密度时，两性IR后直小血管（VR）和PT中的髓质红细胞充血均显著增加（VR：P=0.03；PT：P=0.03）。我们的数据支持以下假设：周细胞密度与SHR中IR损伤的发生呈负相关，这可能由髓质脉管系统中的红细胞充血介导。
