Hashimoto A, Matsumura M, Hirayama K, Fujimoto R, Tanaka K
Faculty of Agriculture and Life Sciences, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori, 036-8561, Japan.
Research Fellow of the Japan Society for the Promotion of Science, 5-3-1 Kojimachi, Chiyoda-ku, Tokyo, 102-0083, Japan.
Stud Mycol. 2017 Jun;87:187-206. doi: 10.1016/j.simyco.2017.07.002. Epub 2017 Jul 13.
The familial placement of four genera, , , , and , was taxonomically revised based on morphological observations and phylogenetic analyses of nuclear rDNA SSU, LSU, , and sequences. ITS sequences were also provided as barcode markers. A total of 130 sequences were newly obtained from 28 isolates which are phylogenetically related to (, ) and its relatives. Phylogenetic analyses and morphological observation of sexual and asexual morphs led to the conclusion that should be restricted to its type genus , which is characterised by ascomata composed of a well-developed, carbonaceous peridium, and an aposphaeria-like coelomycetous asexual morph. Although , , , and are phylogenetically related to , these genera are characterised by epiphyllous, lenticular ascomata with well-developed basal stroma in their sexual morphs, and mycopappus-like propagules in their asexual morphs, which are clearly different from those of . is proposed to accommodate these four genera. Although and have been considered synonyms of based on sexual morphology, we show that they are distinct genera. Based on morphological observations, these genera in are easily distinguished by their synasexual morphs: sigmoid, multi-septate, thin-walled, hyaline conidia (); globose to ovoid, dictyosporus, thick-walled, brown conidia with cellular appendages (); and clavate with a short rostrum, dictyosporus, thick-walled, brown conidia (). A synasexual morph of was not observed. Although was treated as member of in a previous study, it has hyaline cells at the base of ascomata and pseudopycnidial, confluent conidiomata which is atypical features in , and is treated as .
基于对核核糖体DNA小亚基（SSU）、大亚基（LSU）、内转录间隔区（ITS）和线粒体小亚基（mtSSU）序列的形态学观察和系统发育分析，对四个属（ 、 、 和 ）的家族分类进行了修订。ITS序列也作为条形码标记提供。从28个与 （ 、 ）及其近缘种系统发育相关的分离物中总共新获得了130个序列。有性和无性形态的系统发育分析及形态学观察得出结论， 应仅限于其模式属 ，其特征是子囊果由发育良好的碳质包被和类球腔菌状的无性形态组成。虽然 、 、 和 与 在系统发育上相关，但这些属的特征是有性形态为叶生、透镜状子囊果且基部基质发达，无性形态为类霉状繁殖体，这与 的明显不同。建议用 来容纳这四个属。虽然基于有性形态， 和 被认为是 的同义词，但我们表明它们是不同的属。基于形态学观察， 中的这些属通过其准性形态很容易区分：S形、多隔膜、薄壁、透明的分生孢子（ ）；球形至卵形、砖格孢子、厚壁、具细胞附属物的褐色分生孢子（ ）；以及具短喙的棒状、砖格孢子、厚壁、褐色分生孢子（ ）。未观察到 的准性形态。虽然在先前的研究中 被视为 的成员，但它在子囊果基部有透明细胞和假分生孢子器、融合的分生孢子盘，这在 中是非典型特征，因此被视为 。
