Livshits Leonid, Gross Einav
The Hebrew University of Jerusalem, Faculty of Medicine, IMRIC Dept. of Biochemistry & Molecular Biology, P.O. Box 12271, Jerusalem, Israel.
MethodsX. 2017 Jul 19;4:250-255. doi: 10.1016/j.mex.2017.07.005. eCollection 2017.
Cysteine catabolism by gut microbiota produces high levels of sulfide. Excessive sulfide can interfere with colon function, and therefore may be involved in the etiology and risk of relapse of ulcerative colitis, an inflammatory bowel disease affecting millions of people worldwide. Therefore, it is crucial to understand how cells/animals regulate the detoxification of sulfide generated by bacterial cysteine catabolism in the gut. Here we describe a simple and cost-effective way to explore the mechanism of sulfide toxicity in the nematode (). •A rapid cost-effective method to quantify and study sulfide tolerance in and other free-living nematodes.•A cost effective method to measure the concentration of sulfide in the inverted plate assay.
肠道微生物群对半胱氨酸的分解代谢会产生大量的硫化物。过量的硫化物会干扰结肠功能，因此可能与溃疡性结肠炎的病因及复发风险有关，溃疡性结肠炎是一种影响全球数百万人的炎症性肠病。因此，了解细胞/动物如何调节肠道中细菌半胱氨酸分解代谢产生的硫化物的解毒过程至关重要。在此，我们描述了一种简单且经济高效的方法，用于探究线虫中硫化物毒性的机制。•一种快速且经济高效的方法，用于量化和研究秀丽隐杆线虫及其他自由生活线虫对硫化物的耐受性。•一种经济高效的方法，用于在倒平板试验中测量硫化物的浓度。
