使用示差折光检测器通过高效液相色谱法分析生物柴油。

Analysis of biodiesel by high performance liquid chromatography using refractive index detector.

作者信息

Syed Mahin Basha

机构信息

Biochemistry Lab, P.M. Sayeed Calicut University Centre, Androth, U.T. of Lakshadweep, 682551, India.

Department of Chemical Engineering, Annamalai University, Annamalai Nagar, 608002, Tamil Nadu, India.

出版信息

MethodsX. 2017 Jul 19;4:256-259. doi: 10.1016/j.mex.2017.07.002. eCollection 2017.

DOI:10.1016/j.mex.2017.07.002

PMID:28794997

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5547239/

Abstract

High-performance liquid chromatography (HPLC) was used for the determination of compounds occurring during the production of biodiesel from karanja and jatropha oil. Methanol was used for fast monitoring of conversion of karanja and jatropha oil triacylglycerols to fatty acid methyl esters and for quantitation of residual triacylglycerols (TGs), in the final biodiesel product. The individual sample compounds were identified using HPLC. Analysis of fatty acid methyl esters (FAMES) in blends of biodiesel by HPLC using a refractive index and a UV detector at 238 nm. Individual triacylglycerols, diacylglycerols, monoacylglycerols and methyl esters of oleic, linoleic and linolenic acids and free fatty acids were separated within 40 min. Hence HPLC was found to be best for the analysis of biodiesel. Analysis of biodiesel by HPLC using RID detector. Estimation of amount of FAMES in biodiesel. Individual triacylglycerols, diacylglycerols, monoacylglycerols and methyl esters of oleic, linoleic and linolenic acids and free fatty acids were separated within 40 min.

摘要

高效液相色谱法（HPLC）用于测定从麻疯树油和麻风树油生产生物柴油过程中产生的化合物。甲醇用于快速监测麻疯树油和麻风树油三酰甘油向脂肪酸甲酯的转化，并用于定量最终生物柴油产品中残留的三酰甘油（TGs）。使用HPLC鉴定各个样品化合物。通过使用折射率和238nm紫外检测器的HPLC分析生物柴油混合物中的脂肪酸甲酯（FAMEs）。油酸、亚油酸和亚麻酸的三酰甘油、二酰甘油、单酰甘油和甲酯以及游离脂肪酸在40分钟内分离。因此，发现HPLC最适合用于生物柴油分析。使用RID检测器通过HPLC分析生物柴油。估算生物柴油中FAMEs的含量。油酸、亚油酸和亚麻酸的三酰甘油、二酰甘油、单酰甘油和甲酯以及游离脂肪酸在40分钟内分离。