Knabbe C, Lippman M E, Wakefield L M, Flanders K C, Kasid A, Derynck R, Dickson R B
Cell. 1987 Feb 13;48(3):417-28. doi: 10.1016/0092-8674(87)90193-0.
The hormone-dependent human breast cancer cell line MCF-7 secretes transforming growth factor-beta (TGF-beta), which can be detected in the culture medium in a biologically active form. These polypeptides compete with human platelet-derived TGF-beta for binding to its receptor, are biologically active in TGF-beta-specific growth assays, and are recognized and inactivated by TGF-beta-specific antibodies. Secretion of active TGF-beta is induced 8 to 27-fold under treatment of MCF-7 cells with growth inhibitory concentrations of antiestrogens. Antiestrogen-induced TGF-beta from MCF-7 cells inhibits the growth of an estrogen receptor-negative human breast cancer cell line in coculture experiments; growth inhibition is reversed with anti-TGF-beta antibodies. We conclude that in MCF-7 cells, TGF-beta is a hormonally regulated growth inhibitor with possible autocrine and paracrine functions in breast cancer cells.
激素依赖性人乳腺癌细胞系MCF-7分泌转化生长因子-β（TGF-β），它能以生物活性形式在培养基中被检测到。这些多肽与人血小板衍生的TGF-β竞争结合其受体，在TGF-β特异性生长试验中具有生物活性，并被TGF-β特异性抗体识别和灭活。在用生长抑制浓度的抗雌激素处理MCF-7细胞时，活性TGF-β的分泌被诱导增加8至27倍。在共培养实验中，来自MCF-7细胞的抗雌激素诱导的TGF-β抑制雌激素受体阴性的人乳腺癌细胞系的生长；抗TGF-β抗体可逆转生长抑制。我们得出结论，在MCF-7细胞中，TGF-β是一种激素调节的生长抑制剂，在乳腺癌细胞中可能具有自分泌和旁分泌功能。
