You Qiushi, Li Qingqing, Zheng Hailing, Hu Zhiwen, Zhou Yang, Wang Bing
Key Laboratory of Advanced Textile Materials and Manufacturing Technology, Ministry of Education, Zhejiang Sci-Tech University , Hangzhou 310018, China.
Key Scientific Research Base of Textile Conservation, State Administration for Cultural Heritage, China National Silk Museum , Hangzhou 310002, China.
J Agric Food Chem. 2017 Sep 6;65(35):7805-7812. doi: 10.1021/acs.jafc.7b02789. Epub 2017 Aug 22.
Recently, much interest has been paid to the separation of silk produced by Bombyx mori from silk produced by other species and tracing the beginnings of silk cultivation from wild silk exploitation. In this paper, significant differences between silks from Bombyx mori and other species were found by microscopy and spectroscopy, such as morphology, secondary structure, and amino acid composition. For further accurate identification, a diagnostic antibody was designed by comparing the peptide sequences of silks produced by Bombyx mori and other species. The results of the noncompetitive indirect enzyme-linked immunosorbent assay (ELISA) indicated that the antibody that showed good sensitivity and high specificity can definitely discern silk produced by Bombyx mori from silk produced by wild species. Thus, the antibody-based immunoassay has the potential to be a powerful tool for tracing the beginnings of silk cultivation. In addition, combining the sensitive, specific, and convenient ELISA technology with other conventional methods can provide more in-depth and accurate information for species identification.
最近，人们对家蚕所产蚕丝与其他物种所产蚕丝的区分以及从野生蚕丝利用追溯蚕丝养殖的起源产生了浓厚兴趣。在本文中，通过显微镜和光谱学发现家蚕蚕丝与其他物种蚕丝之间存在显著差异，如形态、二级结构和氨基酸组成。为了进一步准确鉴定，通过比较家蚕和其他物种所产蚕丝的肽序列设计了一种诊断抗体。非竞争性间接酶联免疫吸附测定（ELISA）结果表明，该抗体具有良好的敏感性和高特异性，能够明确区分家蚕所产蚕丝与野生物种所产蚕丝。因此，基于抗体的免疫测定法有潜力成为追溯蚕丝养殖起源的有力工具。此外，将灵敏、特异且便捷的ELISA技术与其他传统方法相结合，可以为物种鉴定提供更深入、准确的信息。
