Department of Pharmaceutical Botany, "Iuliu Hațieganu" University of Medicine and Pharmacy, 23, Ghe. Marinescu Street, 400337 Cluj-Napoca, Romania; Institute for Life Sciences, University of Agricultural Sciences and Veterinary Medicine, Calea Mănăştur 3-5, 400372 Cluj-Napoca, Romania.
Department of Biology, Science Faculty, Selcuk University, Konya, Turkey.
Food Chem Toxicol. 2018 Feb;112:448-457. doi: 10.1016/j.fct.2017.08.004. Epub 2017 Aug 8.
This study gathers information about the effects of different extracts (methanol and water) from rhizomes and aerial parts of Iris schachtii on selected enzymes (cholinesterases, alpha-amylase and alpha-glucosidase, tyrosinase and lipase) as well as of their antioxidant capacities and antimutagenic properties in relation with their phenolic composition. The chemical composition was assessed by determining total phenolic and flavonoid content as well as individual phenolic compounds by HPLC-DAD. Moreover, antioxidant abilities of the investigated extracts were tested by using different assays including free radical scavenging (DPPH and ABTS), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating, overall rhizomes being indicated as a superior source of antioxidant compounds. HPLC analysis revealed the abundance of some phenolics including apigenin (2584 μg/g extract) and luteolin (2510 μg/g extract) in aerial parts extracts while rhizomes were rich in apigenin (4734 μg/g extract) and kaempferol (4214 μg/g extract). The methanolic extracts exhibited a high anti-lipase activity while all extracts presented a relatively high inhibition on α-glucosidase. Furthermore, interactions between dominant compounds from extracts and selected enzymes were investigated by molecular modeling studies in order to explain at a molecular level the interactions between selected compounds and active sites of the enzyme/s.
本研究收集了来自鸢尾根茎和地上部分的不同提取物（甲醇和水）对选定酶（胆碱酯酶、α-淀粉酶和α-葡萄糖苷酶、酪氨酸酶和脂肪酶）的影响信息，以及它们的抗氧化能力和抗突变特性与其酚类成分的关系。通过 HPLC-DAD 测定总酚和类黄酮含量以及个别酚类化合物来评估化学成分。此外，还通过使用不同的测定方法（包括 DPPH 和 ABTS 自由基清除、CUPRAC 和 FRAP 还原能力、磷酸钼和金属螯合）测试了所研究提取物的抗氧化能力，结果表明根茎是抗氧化化合物的较好来源。HPLC 分析表明，地上部分提取物中含有一些酚类化合物，包括芹菜素（2584μg/g 提取物）和木犀草素（2510μg/g 提取物），而根茎中富含芹菜素（4734μg/g 提取物）和山奈酚（4214μg/g 提取物）。甲醇提取物表现出较高的抗脂肪酶活性，而所有提取物对α-葡萄糖苷酶的抑制作用都较强。此外，通过分子建模研究研究了提取物中主要化合物与选定酶之间的相互作用，以便从分子水平解释所选化合物与酶/活性位点之间的相互作用。
