College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, China.
Department of Food Science and Nutrition, Zhejiang Key Laboratory of Agro-Food Processing, Zhejiang University, Hangzhou, 310058, China.
Sci Rep. 2017 Aug 10;7(1):7845. doi: 10.1038/s41598-017-08509-6.
We investigated the feasibility and potentiality of determining firmness, soluble solids content (SSC), and pH in kiwifruits using hyperspectral imaging, combined with variable selection methods and calibration models. The images were acquired by a push-broom hyperspectral reflectance imaging system covering two spectral ranges. Weighted regression coefficients (BW), successive projections algorithm (SPA) and genetic algorithm-partial least square (GAPLS) were compared and evaluated for the selection of effective wavelengths. Moreover, multiple linear regression (MLR), partial least squares regression and least squares support vector machine (LS-SVM) were developed to predict quality attributes quantitatively using effective wavelengths. The established models, particularly SPA-MLR, SPA-LS-SVM and GAPLS-LS-SVM, performed well. The SPA-MLR models for firmness (R = 0.9812, RPD = 5.17) and SSC (R = 0.9523, RPD = 3.26) at 380-1023 nm showed excellent performance, whereas GAPLS-LS-SVM was the optimal model at 874-1734 nm for predicting pH (R = 0.9070, RPD = 2.60). Image processing algorithms were developed to transfer the predictive model in every pixel to generate prediction maps that visualize the spatial distribution of firmness and SSC. Hence, the results clearly demonstrated that hyperspectral imaging has the potential as a fast and non-invasive method to predict the quality attributes of kiwifruits.
我们研究了使用高光谱成像技术结合变量选择方法和校准模型来确定猕猴桃硬度、可溶性固形物含量(SSC)和 pH 的可行性和潜力。图像由一个推扫式高光谱反射率成像系统采集,覆盖两个光谱范围。加权回归系数(BW)、连续投影算法(SPA)和遗传算法-偏最小二乘(GAPLS)被用于比较和评估有效波长的选择。此外,采用多元线性回归(MLR)、偏最小二乘回归和最小二乘支持向量机(LS-SVM)建立了基于有效波长的定量预测质量属性的模型。所建立的模型,特别是 SPA-MLR、SPA-LS-SVM 和 GAPLS-LS-SVM 表现良好。SPA-MLR 模型在 380-1023nm 范围内预测硬度(R = 0.9812,RPD = 5.17)和 SSC(R = 0.9523,RPD = 3.26)的性能非常出色,而 GAPLS-LS-SVM 是在 874-1734nm 范围内预测 pH 的最佳模型(R = 0.9070,RPD = 2.60)。开发了图像处理算法,将预测模型从每个像素转移到生成预测图,以可视化硬度和 SSC 的空间分布。因此,结果清楚地表明,高光谱成像技术具有作为一种快速、非侵入性方法来预测猕猴桃质量属性的潜力。