Hirako Ayano, Takeoka Yuki, Furukawa Satoshi, Sugiyama Akihiko
Joint Department of Veterinary Medicine, Faculty of Agriculture, Tottori University, Minami 4-101 Koyama-cho, Tottori, Tottori 680-8553, Japan.
Toxicology and Environmental Science Department, Biological Research Laboratories, Nissan Chemical Industries, Ltd., 1470 Shiraoka, Shiraoka-shi, Saitama 349-0294, Japan.
J Toxicol Pathol. 2017 Jul;30(3):255-260. doi: 10.1293/tox.2017-0015. Epub 2017 May 28.
Adult male medaka () were exposed to 10 ppm of cadmium for 96 h, and the testes were examined histopathologically. Numerous apoptotic cells were found in the spermatogonia and spermatocytes at 72 and 96 h after initiation of cadmium exposure, and the pyknotic index, TUNEL-positive rate, and cleaved caspase-3-positive rate in the spermatogonia and spermatocytes of the cadmium-treated group were higher compared with the control group. No significant difference between the control and cadmium-treated groups was found in the phospho-histone H3-positive rate in the spermatogonia and spermatocytes. No edematous, hemorrhagic, or necrotic changes were observed within the testes in the cadmium-treated group. These results suggest that spermatogonia and spermatocytes in medaka testes are highly sensitive to cadmium. Exposure to 10 ppm of cadmium induced histopathologic changes in the testes that were similar to those described in rodents exposed to low doses of cadmium.
将成年雄性青鳉鱼暴露于10 ppm的镉中96小时，然后对其睾丸进行组织病理学检查。在镉暴露开始后72小时和96小时，精原细胞和精母细胞中发现大量凋亡细胞，与对照组相比，镉处理组精原细胞和精母细胞中的固缩指数、TUNEL阳性率和裂解的caspase-3阳性率更高。精原细胞和精母细胞中磷酸化组蛋白H3阳性率在对照组和镉处理组之间未发现显著差异。镉处理组睾丸内未观察到水肿、出血或坏死变化。这些结果表明，青鳉鱼睾丸中的精原细胞和精母细胞对镉高度敏感。暴露于10 ppm的镉会引起睾丸组织病理学变化，这些变化与低剂量镉暴露的啮齿动物中描述的变化相似。
