Yu Yang, Li Quan-Feng, Zhang Jin-Ping, Zhang Fan, Zhou Yan-Fei, Feng Yan-Zhao, Chen Yue-Qin, Zhang Yu-Chan
State Key Laboratory for Biocontrol, School of Life Science, Sun Yat-sen UniversityGuangzhou, China.
Front Plant Sci. 2017 Jul 26;8:1324. doi: 10.3389/fpls.2017.01324. eCollection 2017.
Seed setting rate is one of the most important components of rice grain yield. To date, only several genes regulating setting rate have been identified in plant. In this study, we showed that (), a member of laccase family genes which are known for their roles in modulating phenylpropanoid pathway and secondary lignification in cell wall, exerts a regulatory function in rice seed setting rate. expressed in anthers and promotes hydrogen peroxide production both and in the filaments and anther connectives. Knock-out of showed significantly increased seed setting rate, while overexpression of this gene exhibited induced mitochondrial damage and suppressed sugar transportation in anthers, which in turn affected seed setting rate. also induced HO production and mitochondrial damage in the root tip cells which caused the lethal phenotype. We also showed that high abundant of OsmiR397, the suppressor of mRNA, increased the seed setting rate of rice plants, and restrains HO accumulation in roots during oxidative stress. Our results suggested a novel regulatory role of gene in regulating seed setting rate by affecting HO dynamics and mitochondrial integrity in rice.
结实率是水稻产量最重要的组成部分之一。迄今为止，在植物中仅鉴定出几个调控结实率的基因。在本研究中，我们发现漆酶家族基因成员（），其以在调节苯丙烷途径和细胞壁次生木质化中的作用而闻名，在水稻结实率中发挥调控功能。在花药中表达，并在花丝和花药连接组织中促进过氧化氢的产生。敲除显示结实率显著提高，而该基因的过表达表现出诱导线粒体损伤并抑制花药中的糖分运输，进而影响结实率。还诱导根尖细胞中HO的产生和线粒体损伤，导致致死表型。我们还表明，作为mRNA抑制剂的OsmiR397含量高会提高水稻植株的结实率，并在氧化应激期间抑制根中HO的积累。我们的结果表明基因通过影响水稻中HO动态和线粒体完整性在调控结实率方面具有新的调控作用。
