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肿瘤蛋白D52和D54对软骨细胞的终末分化具有相反作用。

Tumor Proteins D52 and D54 Have Opposite Effects on the Terminal Differentiation of Chondrocytes.

作者信息

Ito Chihiro, Mukudai Yoshiki, Itose Masakatsu, Kato Kosuke, Motohashi Hiromi, Shimane Toshikazu, Kondo Seiji, Shirota Tatsuo

机构信息

Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ota-ku, Tokyo 145-8515, Japan.

Department of Biochemistry, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.

出版信息

Biomed Res Int. 2017;2017:6014278. doi: 10.1155/2017/6014278. Epub 2017 Jul 17.

DOI:10.1155/2017/6014278
PMID:28798933
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5535702/
Abstract

The tumor protein D (TPD) family consists of four members, TPD52, TPD53, TPD54, and TPD55. The physiological roles of these genes in normal tissues, including epidermal and mesenchymal tissues, have rarely been reported. Herein, we examined the expression of TPD52 and TPD54 genes in cartilage in vivo and in vitro and investigated their involvement in the proliferation and differentiation of chondrocytes in vitro. TPD52 and TPD54 were uniformly expressed in articular cartilage and trabecular bone and were scarcely expressed in the epiphyseal growth plate. In MC3T3E-1 cells, the expressions of TPD52 and TPD54 were increased in a differentiation-dependent manner. In contrast, their expressions were decreased in ATDC5 cells. In ATDC5 cells, overexpression of TPD52 decreased alkaline phosphatase (ALPase) activity, while knock-down of TPD52 showed little effect. In contrast, overexpression of TPD54 enhanced ALPase activity, Ca deposition, and the expressions of type X collagen and ALPase genes, while knock-down of TPD54 reduced them. The results revealed that TPD52 inhibits and that TPD54 promotes the terminal differentiation of a chondrocyte cell line. As such, we report for the first time the important roles of TPD52 and TPD54, which work oppositely, in the terminal differentiation of chondrocytes during endochondral ossification.

摘要

肿瘤蛋白D（TPD）家族由四个成员组成，即TPD52、TPD53、TPD54和TPD55。这些基因在包括表皮和间充质组织在内的正常组织中的生理作用鲜有报道。在此，我们检测了TPD52和TPD54基因在体内外软骨中的表达，并研究了它们在体外软骨细胞增殖和分化中的作用。TPD52和TPD54在关节软骨和松质骨中均匀表达，而在骨骺生长板中几乎不表达。在MC3T3E-1细胞中，TPD52和TPD54的表达以分化依赖的方式增加。相反，它们在ATDC5细胞中的表达降低。在ATDC5细胞中，TPD52的过表达降低了碱性磷酸酶（ALPase）活性，而敲低TPD52则几乎没有影响。相反，TPD54的过表达增强了ALPase活性、钙沉积以及X型胶原蛋白和ALPase基因的表达，而敲低TPD54则使其降低。结果表明，TPD52抑制而TPD54促进软骨细胞系的终末分化。因此，我们首次报道了在软骨内骨化过程中，作用相反的TPD52和TPD54在软骨细胞终末分化中的重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78c3/5535702/c74e7c4b1c43/BMRI2017-6014278.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78c3/5535702/7d2c2cf932b4/BMRI2017-6014278.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78c3/5535702/557c44403c25/BMRI2017-6014278.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78c3/5535702/04611eb4b8cb/BMRI2017-6014278.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78c3/5535702/a9c1bf28a1c4/BMRI2017-6014278.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78c3/5535702/7234264b625c/BMRI2017-6014278.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78c3/5535702/c74e7c4b1c43/BMRI2017-6014278.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78c3/5535702/7d2c2cf932b4/BMRI2017-6014278.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78c3/5535702/557c44403c25/BMRI2017-6014278.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78c3/5535702/04611eb4b8cb/BMRI2017-6014278.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78c3/5535702/a9c1bf28a1c4/BMRI2017-6014278.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78c3/5535702/7234264b625c/BMRI2017-6014278.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/78c3/5535702/c74e7c4b1c43/BMRI2017-6014278.006.jpg

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2
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Tumour Biol. 2014 Aug;35(8):7369-82. doi: 10.1007/s13277-014-2006-x. Epub 2014 May 6.
3
Potential anti-osteoporotic effects of herbal extracts on osteoclasts, osteoblasts and chondrocytes in vitro.草药提取物对体外破骨细胞、成骨细胞和软骨细胞的潜在抗骨质疏松作用。
卵巢癌细胞中肿瘤蛋白 D52（TPD52）与 KLF9、PKCε 和 MicroRNA 223 的串扰。
J Ovarian Res. 2023 Oct 13;16(1):202. doi: 10.1186/s13048-023-01292-1.
4
Predicting 3D Structure, Cross Talks, and Prognostic Significance of in Cervical Cancer.预测宫颈癌中[具体内容未给出]的三维结构、相互作用及预后意义。
Front Oncol. 2022 Jan 3;11:797007. doi: 10.3389/fonc.2021.797007. eCollection 2021.
5
The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer.肿瘤蛋白D54在调节丙酮酸脱氢酶及二甲双胍对乳腺癌细胞毒性中的新功能。
Cancer Metab. 2019 Jan 24;7:1. doi: 10.1186/s40170-018-0193-4. eCollection 2019.
BMC Complement Altern Med. 2014 Jan 17;14:29. doi: 10.1186/1472-6882-14-29.
4
Tumor protein D54 is a negative regulator of extracellular matrix-dependent migration and attachment in oral squamous cell carcinoma-derived cell lines.肿瘤蛋白 D54 是口腔鳞状细胞癌细胞系中细胞外基质依赖的迁移和黏附的负调节剂。
Cell Oncol (Dordr). 2013 Jun;36(3):233-45. doi: 10.1007/s13402-013-0131-y. Epub 2013 Mar 26.
5
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6
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