p1220-CTXM，一种与pKP048相关的携带bla和qnrB4的IncFII质粒。
p1220-CTXM, a pKP048-related IncFII plasmid carrying bla and qnrB4.
作者信息
Zhang Defu, Yin Zhe, Zhao Yuzong, Feng Jiao, Jiang Xiaoyuan, Zhan Zhe, Wu Weili, Chen Weijun, Wang Jinglin, Li Jianrong, Zhou Dongsheng
机构信息
State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
College of Food Science & Project Engineering, Bohai University, Jinzhou 121013, China.
出版信息
Future Microbiol. 2017 Sep;12:1035-1043. doi: 10.2217/fmb-2017-0026. Epub 2017 Aug 11.
AIM
This study aimed to characterize plasmid-mediated antimicrobial resistance in clinical Klebsiella pneumoniae 1220 carrying bla and qnrB4.
MATERIALS & METHODS: Plasmid p1220-CTXM was transformed from the 1220 isolate into Escherichia coli through conjugal transfer and then fully sequenced. Antimicrobial susceptibility was determined by VITEK.
RESULTS
p1220-CTXM was an IncFII plasmid genetically closely related to pKP048 and carried resistance markers including bla , bla , qnrB4, sul1 and qacEΔ1, all of which were harbored in a 35.7-kb multidrug-resistant region. bla was located in a truncated ISEcp1-bla -orf477 transposition unit, and qnrB4 and bla were in a truncated qnrB4-bla region.
CONCLUSION
This study provided the insight into the co-occurrence of bla and qnrB4 and the evolution of pKP048-related IncFII plasmids.
目的
本研究旨在对携带bla和qnrB4的临床肺炎克雷伯菌1220中的质粒介导的抗菌药物耐药性进行表征。
材料与方法
通过接合转移将质粒p1220-CTXM从1220分离株转化到大肠杆菌中，然后进行全测序。采用VITEK测定抗菌药物敏感性。
结果
p1220-CTXM是一种IncFII质粒，与pKP048在基因上密切相关，并携带耐药标记，包括bla、bla、qnrB4、sul1和qacEΔ1，所有这些都位于一个35.7 kb的多药耐药区域。bla位于一个截短的ISEcp1-bla-orf477转座单元中，qnrB4和bla位于一个截短的qnrB4-bla区域。
结论
本研究为bla和qnrB4的共现以及pKP048相关IncFII质粒的进化提供了见解。
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