Zhang Defu, Yin Zhe, Zhao Yuzong, Feng Jiao, Jiang Xiaoyuan, Zhan Zhe, Wu Weili, Chen Weijun, Wang Jinglin, Li Jianrong, Zhou Dongsheng
State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.
College of Food Science & Project Engineering, Bohai University, Jinzhou 121013, China.
Future Microbiol. 2017 Sep;12:1035-1043. doi: 10.2217/fmb-2017-0026. Epub 2017 Aug 11.
This study aimed to characterize plasmid-mediated antimicrobial resistance in clinical Klebsiella pneumoniae 1220 carrying bla and qnrB4.
MATERIALS & METHODS: Plasmid p1220-CTXM was transformed from the 1220 isolate into Escherichia coli through conjugal transfer and then fully sequenced. Antimicrobial susceptibility was determined by VITEK.
p1220-CTXM was an IncFII plasmid genetically closely related to pKP048 and carried resistance markers including bla , bla , qnrB4, sul1 and qacEΔ1, all of which were harbored in a 35.7-kb multidrug-resistant region. bla was located in a truncated ISEcp1-bla -orf477 transposition unit, and qnrB4 and bla were in a truncated qnrB4-bla region.
This study provided the insight into the co-occurrence of bla and qnrB4 and the evolution of pKP048-related IncFII plasmids.
本研究旨在对携带bla和qnrB4的临床肺炎克雷伯菌1220中的质粒介导的抗菌药物耐药性进行表征。
通过接合转移将质粒p1220-CTXM从1220分离株转化到大肠杆菌中，然后进行全测序。采用VITEK测定抗菌药物敏感性。
p1220-CTXM是一种IncFII质粒，与pKP048在基因上密切相关，并携带耐药标记，包括bla、bla、qnrB4、sul1和qacEΔ1，所有这些都位于一个35.7 kb的多药耐药区域。bla位于一个截短的ISEcp1-bla-orf477转座单元中，qnrB4和bla位于一个截短的qnrB4-bla区域。
本研究为bla和qnrB4的共现以及pKP048相关IncFII质粒的进化提供了见解。
