Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Centre for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, PR China.
Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Centre for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications, 9 Wenyuan Road, Nanjing 210023, PR China; Department of Medical Imaging, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, PR China; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, PR China.
J Colloid Interface Sci. 2017 Dec 1;507:242-249. doi: 10.1016/j.jcis.2017.08.008. Epub 2017 Aug 3.
In this work, we report a facile biphasic-to-monophasic successive co-assembly approach to synthesize yolk-shell structured mesoporous organosilica nanoparticles (MONs). The yolk-shell structured MONs possess ethane-bridged frameworks, high surface area (1023mg), radially oriented mesochannels (3.8nm), large pore volume (0.99cmg), and tunable diameter (147-324nm) and shell thickness (23-53nm). The biphasic-to-monophasic successive co-assembly method is intrinsically simple and requires neither sacrificial templates nor multistep coating processes. The key of the method is that the interiors of the mesostructured organosilica nanospheres grown in the biphasic system have a lower condensation degree and Si-C-C-Si species content than the outer shells formed in the monophasic system. Thus, the interior layer is attracted by OH anions and dissolved in the monophasic system, forming the yolk-shell structures. In vitro cytotoxicity and haemolysis assays demonstrate that the ethane-bridged yolk-shell MONs possess excellent biocompatibility. Furthermore, the chemotherapy drug doxorubicin (DOX) is loaded into the yolk-shell MONs to kill drug-resistant MCF-7/ADR human breast cancer cells. Compared with free DOX and DOX-loaded typical MONs, the DOX-loaded yolk-shell MONs have higher chemotherapeutic efficacy against MCF-7/ADR cells, suggesting the great potential of yolk-shell MONs synthesized via the biphasic-to-monophasic successive co-assembly approach in the biomedical field.
在这项工作中，我们报告了一种简便的双相反连续共组装方法来合成蛋黄壳结构的介孔有机硅纳米粒子（MONs）。蛋黄壳结构的 MONs 具有乙烷桥接骨架、高比表面积（1023mg）、径向取向的介孔（3.8nm）、大孔体积（0.99cmg）和可调直径（147-324nm）和壳厚（23-53nm）。双相反连续共组装方法本质上简单，既不需要牺牲模板，也不需要多步涂覆过程。该方法的关键是在双相反相中生长的介孔有机硅纳米球的内部具有比单相系统中形成的外壳更低的缩合度和 Si-C-C-Si 物质含量。因此，内层被 OH 阴离子吸引并溶解在单相系统中，形成蛋黄壳结构。体外细胞毒性和溶血试验表明，乙烷桥接蛋黄壳 MONs 具有优异的生物相容性。此外，将化疗药物阿霉素（DOX）负载到蛋黄壳 MONs 中以杀死耐药 MCF-7/ADR 人乳腺癌细胞。与游离 DOX 和负载 DOX 的典型 MONs 相比，负载 DOX 的蛋黄壳 MONs 对 MCF-7/ADR 细胞具有更高的化疗疗效，表明通过双相反连续共组装方法合成的蛋黄壳 MONs 在生物医学领域具有巨大的潜力。
