Song Wen, Peng Man, Duan Shi-Yu, Lin Chuang, Xu Qiong, Zhou Jun
Department of Pathology, Nanfang Hospital1, Department of Radiotherapy, Nanfang Hospital2, Department of Pathology, School of Basic Medical Sciences3, Southern Medical University, Guangzhou 510515, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2017 Aug 20;37(8):1040-1046. doi: 10.3969/j.issn.1673-4254.2017.08.07.
To explore role of MIER3 gene in the development and progression of human colorectal carcinoma (CRC) and analyze the proteins that interact with MIER3 using bioinformatic techniques.
MIER3 mRNA and protein expressions were detected in 8 CRC biopsy samples and paired adjacent tissues using real-time PCR and Western blotting. A recombinant eukaryotic expression vector pcDNA3-MIER3 was constructed and its effect on the proliferation and invasion of CRC cells were tested using CCK8 assay and Transwell migration assay. Bioinformatic methods were used to predict and analyze MIER3-interacting proteins.
MIER3 was obviously down-regulated in the 8 CRC tissues as compared with the paired adjacent tissues. In human CRC cell line DLD1, MIER3 overexpression induced by transfection of the cells with pcDNA3-MIER3 significantly inhibited the cell proliferation and suppressed cell invasiveness in vitro. Bioinformatics analyses indicated that NAT9 was a potential MIER3-interacting protein and MIER3 was probably associated with tumor susceptibility.
MIER3, which is obviously down-regulated in CRC tissues, is closely associated with the proliferation and invasion of CRC, and NAT9 protein is a probable MIER3-interacting protein.
探讨MIER3基因在人类结直肠癌（CRC）发生发展及进程中的作用，并运用生物信息学技术分析与MIER3相互作用的蛋白质。
采用实时定量PCR和蛋白质免疫印迹法检测8例CRC活检样本及其配对的癌旁组织中MIER3 mRNA和蛋白质的表达。构建重组真核表达载体pcDNA3-MIER3，运用CCK8法和Transwell迁移实验检测其对CRC细胞增殖和侵袭的影响。采用生物信息学方法预测和分析与MIER3相互作用的蛋白质。
与配对的癌旁组织相比，8例CRC组织中MIER3明显下调。在人CRC细胞系DLD1中，用pcDNA3-MIER3转染细胞诱导MIER3过表达，显著抑制细胞增殖并在体外抑制细胞侵袭性。生物信息学分析表明NAT9是一种潜在的与MIER3相互作用的蛋白质，且MIER3可能与肿瘤易感性相关。
MIER3在CRC组织中明显下调，与CRC的增殖和侵袭密切相关，NAT9蛋白可能是与MIER3相互作用的蛋白质。
