Yang Hyung-Seok, Kim Youngjin, Cho Sun Young, Nam You Sun, Oh Tae Sang, Park Kyung-Sun, Kim Hyun-Chul, Lee Hee Joo
Department of Laboratory Medicine, School of Medicine, Kyung Hee University, Seoul, Korea.
Department of Biomedical Science, Graduate School, Kyung Hee University, Seoul, Korea.
Ann Clin Lab Sci. 2017 Aug;47(4):511-515.
To investigate the sequence types (STs) and fluoroquinolone resistance related mutations among ciprofloxacin (CIP)-non-susceptible extended-spectrum β-lactamase (ESBL)-producing isolated from Korean patients from 2006-2008. The prevalence of fluoroquinolone resistance-determining region (QRDR) mutations in , and and plasmid-mediated quinolone resistance (PMQR) genes were also studied. Multilocus sequence typing (MLST) was performed to identify STs. The most common ST was ST131 (33/51, 64.7%). All isolates, except one isolate, showed three mutations at codons 83 (S83L) and 87 (S87N) in and 80 (S80I) in The prevalence of ST131 in our hospital was much higher than reported in other Asian studies during a similar time period. The mutations found in ST131 were concordant with other studies.
为调查2006 - 2008年从韩国患者中分离出的对环丙沙星（CIP）不敏感且产超广谱β-内酰胺酶（ESBL）的菌株的序列类型（STs）以及与氟喹诺酮耐药相关的突变。还研究了gyrA、gyrB、parC和parE中氟喹诺酮耐药决定区（QRDR）突变的发生率以及质粒介导的喹诺酮耐药（PMQR）基因。采用多位点序列分型（MLST）来鉴定STs。最常见的ST是ST131（33/51，64.7%）。除一株分离株外，所有分离株在gyrA的第83位密码子（S83L）和第87位密码子（S87N）以及parC的第80位密码子（S80I）处均出现三个突变。我院ST131的发生率远高于同期其他亚洲研究报道的发生率。在ST131中发现的突变与其他研究结果一致。
