Gould David
Department of Biochemical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London, EC1M 6BQ, UK.
Methods Mol Biol. 2017;1651:1-7. doi: 10.1007/978-1-4939-7223-4_1.
Before designing a synthetic promoter, it can be helpful to think about its final application. Is the study purely an in vitro exercise in monitoring short-term promoter activity from an episomal vector, or does the promoter eventually need to be permanently active and be integrated into the genome or perhaps even to function in vivo? The final application will have a bearing on promoter design and vector of choice from the start of the study. In this chapter I highlight some of the vector attributes to consider and features that should be thought about.
在设计合成启动子之前，考虑其最终应用可能会有所帮助。该研究仅仅是一项体外实验，用于监测游离型载体的短期启动子活性，还是该启动子最终需要永久激活并整合到基因组中，甚至可能在体内发挥作用？从研究开始，最终应用就会对启动子设计和所选载体产生影响。在本章中，我将重点介绍一些需要考虑的载体属性和应予以关注的特征。
