通过电泳迁移率变动分析证明转录因子与DNA的相互作用。

Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay.

作者信息

Yousaf Nasim, Gould David

机构信息

Bone and Joint Research Unit, William Harvey Research Institute, Queen Mary University of London, London, EC1M 6BQ, UK.

Department of Biochemical Pharmacology, William Harvey Research Institute, Queen Mary University of London, London, EC1M 6BQ, UK.

出版信息

Methods Mol Biol. 2017;1651:11-21. doi: 10.1007/978-1-4939-7223-4_2.

DOI:10.1007/978-1-4939-7223-4_2

PMID:28801896

Abstract

Confirming the binding of a transcription factor with a particular DNA sequence may be important in characterizing interactions with a synthetic promoter. Electrophoretic mobility shift assay is a powerful approach to demonstrate the specific DNA sequence that is bound by a transcription factor and also to confirm the specific transcription factor involved in the interaction. In this chapter we describe a method we have successfully used to demonstrate interactions of endogenous transcription factors with sequences derived from endogenous and synthetic promoters.

摘要

证实转录因子与特定DNA序列的结合对于表征其与合成启动子的相互作用可能很重要。电泳迁移率变动分析是一种强大的方法，可用于证明转录因子所结合的特定DNA序列，并确认参与该相互作用的特定转录因子。在本章中，我们描述了一种我们已成功用于证明内源性转录因子与源自内源性和合成启动子的序列之间相互作用的方法。