超高效液相色谱-紫外检测法和超高效液相色谱-电喷雾离子化-四极杆-飞行时间质谱法研究马齿苋中油酸酰胺A在大鼠体内的药代动力学和代谢情况
Pharmacokinetics and metabolism of olerciamide A from Portulaca oleracea L. in rats by UHPLC-UV and UHPLC-ESI-Q-TOF/MS.
作者信息
Ying Zheming, Li Cuiyu, Gao Mingzhe, Ying Xixiang, Yang Guanlin
机构信息
School of The First Clinic, Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning, People's Republic of China.
School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian, Liaoning, People's Republic of China.
出版信息
Biomed Chromatogr. 2018 Feb;32(2). doi: 10.1002/bmc.4061. Epub 2017 Sep 10.
The aim of this study was to elucidate the pharmacokinetics of olerciamide A in rats after oral and intravenous administration of Portulaca oleracea L. extract by a simple and rapid ultra high-performance liquid chromatography method with bergapten as internal standard. The pharmacokinetic results indicated that olerciamide A was rapidly distributed with a time to peak concentration of 30 min after oral administration and presented a low oral absolute bioavailability of 4.57%. The metabolism of olerciamide A in rats was also investigated using ultra-high-performance liquid chromatography electrospray coupled with quadrupole-time of flight mass spectrometry to elucidate the reason for the low absolute bioavailability of olerciamide A and seven metabolites of oleraciamide A were found in rat plasma and urine.
本研究的目的是通过一种简单快速的超高效液相色谱法，以补骨脂素为内标，阐明马齿苋提取物经口服和静脉给药后，大鼠体内油酰胺A的药代动力学。药代动力学结果表明，油酰胺A分布迅速，口服给药后达峰时间为30分钟，口服绝对生物利用度低，为4.57%。还采用超高效液相色谱-电喷雾-四极杆飞行时间质谱联用技术研究了油酰胺A在大鼠体内的代谢情况，以阐明油酰胺A绝对生物利用度低的原因，并在大鼠血浆和尿液中发现了7种油酰胺A的代谢产物。
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