Detection of Mycobacterium tuberculosis from paraffin-embedded tissues by GeneXpert MTB/RIF.

作者信息

Rindi Laura, Alì Greta, Fabiani Barbara, Fontanini Gabriella, Garzelli Carlo

机构信息

Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, I-56127, Pisa, Italy.

Unit of Pathological Anatomy, University Hospital of Pisa, I-56126, Pisa, Italy.

出版信息

Tuberculosis (Edinb). 2017 Sep;106:53-55. doi: 10.1016/j.tube.2017.06.005. Epub 2017 Jul 3.

GeneXpert MTB/RIF (Xpert) assay, a rapid and automated system based on real-time PCR and molecular beacon technology, proved to be a sensitive and specific tool capable of detecting Mycobacterium tuberculosis and rifampin resistance in clinical specimens. In this study we provide a Xpert-dedicated successful protocol for processing paraffin-embedded tissue and assess the feasibility of the Xpert assay-based tuberculosis (TB) diagnosis on these specimens, thus proving the Xpert assay as a valuable TB diagnostic tool in supporting conventional histopathological methods.