Dunlap E D, Samols E, Waite L C, Pfeifer M A
Metabolism. 1987 Feb;36(2):193-7. doi: 10.1016/0026-0495(87)90017-5.
A noninvasive method to evaluate autonomic nervous system (ANS) function in animals is needed for studies of diabetic autonomic neuropathy. These studies modified the RR-variation test, used to test diabetic ANS function in humans, and applied it to rats. Permanent wire electrodes were implanted in the chest wall of a rat. ECG complexes were obtained by connecting the electrodes to leads going to an impedence pneumograph and high gain coupler. This information was then converted into square waves by a trigger unit and recorded on magnetic tape for subsequent analysis by computer. Recordings were at least 60 seconds long, of which 30 seconds was used for analysis. In order to establish autonomic influence, RR-variation was measured before and after application of pharmacologic agents. Directly decreasing parasympathetic tone with atropine (20 mg/kg, n = 6) increased heart rate (P less than 0.001) and decreased RR-variation (P less than 0.05). Directly decreasing beta adrenergic tone with propranolol (10 mg/kg, n = 7) decreased heart rate (P less than 0.01) but had no effect on RR-variation (NS). Stimulation of the beta adrenergic receptors (isoproterenol, 0.1 mg/kg, n = 5) increased heart rate (P less than 0.01) but decreased RR-variation (P less than 0.01). Increasing parasympathetic tone reflexly with alpha-1 adrenergic receptor stimulation (phenylephrine, 1 mg/kg, n = 7) decreased heart rate (P less than 0.05) and increased RR-variation (P less than 0.025). The responses to phenylephrine could be blocked by parasympathetic blockade. Phentolamine (0.1 mg/kg, n = 7) caused an increase in heart rate (P less than 0.001) and a decrease in RR-variation (P less than 0.01). The responses to phentolamine could be blocked by beta adrenergic receptor blockade.(ABSTRACT TRUNCATED AT 250 WORDS)
糖尿病自主神经病变的研究需要一种评估动物自主神经系统（ANS）功能的非侵入性方法。这些研究对用于检测人类糖尿病ANS功能的RR变异试验进行了改进，并将其应用于大鼠。将永久性线状电极植入大鼠胸壁。通过将电极连接到通向阻抗呼吸描记器和高增益耦合器的导联来获取心电图复合波。然后，该信息由触发单元转换为方波，并记录在磁带上以供计算机后续分析。记录至少持续60秒，其中30秒用于分析。为了确定自主神经的影响，在应用药物前后测量RR变异。用阿托品（20mg/kg，n = 6）直接降低副交感神经张力会使心率增加（P < 0.001），RR变异降低（P < 0.05）。用普萘洛尔（10mg/kg，n = 7）直接降低β肾上腺素能张力会使心率降低（P < 0.01），但对RR变异无影响（无显著性差异）。刺激β肾上腺素能受体（异丙肾上腺素，0.1mg/kg，n = 5）会使心率增加（P < 0.01），但RR变异降低（P < 0.01）。用α-1肾上腺素能受体刺激（去氧肾上腺素，1mg/kg，n = 7）反射性增加副交感神经张力会使心率降低（P < 0.05），RR变异增加（P < 0.025）。对去氧肾上腺素的反应可被副交感神经阻滞所阻断。酚妥拉明（0.1mg/kg，n = 7）会使心率增加（P < 0.001），RR变异降低（P < 0.01）。对酚妥拉明的反应可被β肾上腺素能受体阻滞所阻断。（摘要截取自250字）
