Department of Chemistry, ‡Center for Bioactive Delivery-Institute for Applied Life Sciences, and §Molecular and Cellular Biology Program, University of Massachusetts , Amherst, Massachusetts 01003, United States.
Langmuir. 2017 Dec 12;33(49):14004-14010. doi: 10.1021/acs.langmuir.7b02488. Epub 2017 Aug 31.
Supramolecular reverse micelle assemblies, formed by amphiphilic copolymers, can selectively encapsulate molecules in their interiors depending on the functional groups present in the polymers. Altering the binding selectivity of these materials typically requires the synthesis of alternate functional groups. Here, we demonstrate that the addition of Zr(IV) ions to the interiors of reverse micelles having phosphonate functional groups transforms the supramolecular materials from ones that selectively bind positively charged peptides into materials that selectively bind phosphorylated peptides. We also show that the binding selectivity of these reverse micelle assemblies can be further tuned by varying the fractions of phosphonate groups in the copolymer structure. The optimized reverse micelle materials can selectively transfer and bind phosphorylated peptides from aqueous solutions over a wide range of pH conditions and can selectively enrich phosphorylated peptides even in complicated mixtures.
超分子反向胶束组装体由两亲性共聚物形成，可根据聚合物中存在的官能团选择性地将分子包裹在内部。改变这些材料的结合选择性通常需要合成不同的官能团。在这里，我们证明向具有膦酸根基团的反向胶束内部添加 Zr(IV) 离子，可以将超分子材料从选择性结合正电荷肽的材料转变为选择性结合磷酸化肽的材料。我们还表明，通过改变共聚物结构中膦酸根基团的比例，可以进一步调整这些反向胶束组装体的结合选择性。优化后的反向胶束材料可以在广泛的 pH 条件下从水溶液中选择性地转移和结合磷酸化肽，甚至可以在复杂混合物中选择性地富集磷酸化肽。
