Singh Pradeep Kumar, Yadav Vivek Kumar, Kalia Manmohit, Dohare Suhaga, Sharma Deepmala, Agarwal Vishnu
Department of Biotechnology, Motilal Nehru National Institute of Technology, Allahabad, India.
Department of Mathematics, National Institute of Technology, Raipur, India.
Microb Pathog. 2017 Sep;110:612-619. doi: 10.1016/j.micpath.2017.08.009. Epub 2017 Aug 10.
Pseudomonas aeruginosa has evolved the 3-oxo-C-HSL and C-HSL based quorum sensing system which is responsible for the regulation of various virulence factors and helps to dominates over other bacterial species. Staphylococcus epidermidis has frequently been reported with P. aeruginosa while the role of C-HSL and 3-oxo-C-HSL on the S. epidermidis had widely been unexplored, and as per our knowledge, this is the first report on the impact of C-HSL and 3-oxo-C-HSL overS. epidermidis growth and biofilm. We found that among the two AHL molecules; only 3-oxo-C-HSL was able to exert a significant effect in all the experiments including growth and biofilm of S. epidermidis. 3-oxo-C-HSL at 100 μM and 200 μM concentrations were able to initiate the apparent transient type of planktonic growth inhibition in S. epidermidis. Microscopic analysis and biofilm quantification assay showed the inhibitory effect of 3-oxo-C-HSL against S. epidermidis biofilm, initial attachment, and EPS production. The study concludes that P. aeruginosa associated 3-oxo-C-HSL exerts the inhibitory effect on S. epidermidis growth and biofilms and thus it may also help Pseudomonasto dominate under the co-infection conditions.
铜绿假单胞菌进化出了基于3-氧代-C-高丝氨酸内酯（3-oxo-C-HSL）和C-高丝氨酸内酯（C-HSL）的群体感应系统，该系统负责调节各种毒力因子，并有助于在其他细菌物种中占据主导地位。表皮葡萄球菌经常与铜绿假单胞菌一起被报道，而C-HSL和3-oxo-C-HSL对表皮葡萄球菌的作用在很大程度上尚未得到探索，据我们所知，这是关于C-HSL和3-oxo-C-HSL对表皮葡萄球菌生长和生物膜影响的首次报道。我们发现，在这两种AHL分子中，只有3-oxo-C-HSL能够在包括表皮葡萄球菌生长和生物膜在内的所有实验中发挥显著作用。100μM和200μM浓度的3-oxo-C-HSL能够引发表皮葡萄球菌明显的短暂性浮游生长抑制。显微镜分析和生物膜定量分析显示了3-oxo-C-HSL对表皮葡萄球菌生物膜、初始附着和胞外多糖产生的抑制作用。该研究得出结论，铜绿假单胞菌相关的3-oxo-C-HSL对表皮葡萄球菌的生长和生物膜具有抑制作用，因此它也可能有助于铜绿假单胞菌在共感染条件下占据主导地位。
