Resta L, Zito F A, Maiorano E, Pollice L
Tumori. 1986 Dec 31;72(6):629-36. doi: 10.1177/030089168607200616.
Cells with argyrophil granules were searched for in 131 consecutive specimens obtained from endometrial curettage. Particular care was taken with the fixation methods to avoid a false positivity to the silver impregnation according to Grimelius. We did not consider the grossly argyrophilic positivity on the cellular apex or of the whole cytoplasm, which was partially reduced by diastase digestion and probably due to the presence of glycogen and secretory mucoproteins. Cells with thin argyrophilic granules similar to those observed in endocrine APUD cells were present among glandular cells and/or among stromal cells in 15 of 131 examined specimens. They concerned 5 cases of proliferative endometrium, 3 of secretive endometrium, 5 of hyperplasia, and 2 of carcinoma. In 8 of the 15 cases with argyrophilic cells, immunohistochemical studies with the PAP method showed cells with the presence of FSH, S-100 protein, somatostatin, vasoactive intestinal peptide (VIP), gastrin, and neuron-specific enolase (NSE). The significance and origin of these cells in normal and neoplastic endometrium are discussed.
在131例连续的子宫内膜刮除标本中寻找嗜银颗粒细胞。根据格里梅利乌斯法，特别注意固定方法以避免对银浸染出现假阳性。我们不考虑细胞顶端或整个细胞质的明显嗜银阳性，这种阳性经淀粉酶消化后会部分减弱，可能是由于糖原和分泌性粘蛋白的存在。在131例检查标本中的15例中，腺细胞和/或基质细胞中存在与内分泌APUD细胞中观察到的类似的细嗜银颗粒细胞。它们涉及5例增殖期子宫内膜、3例分泌期子宫内膜、5例增生和2例癌。在15例有嗜银细胞的病例中的8例中，用PAP法进行的免疫组织化学研究显示细胞存在促卵泡激素、S-100蛋白、生长抑素、血管活性肠肽（VIP）、胃泌素和神经元特异性烯醇化酶（NSE）。讨论了这些细胞在正常和肿瘤性子宫内膜中的意义和来源。
