遗传性血栓形成倾向的评估：蛋白C（PC）检测的性能

Assessment of Hereditary Thrombophilia: Performance of Protein C (PC) Testing.

作者信息

Marlar Richard A, Gausman Jana N

机构信息

Department of Pathology, University of New Mexico, Albuquerque, NM, 87131, USA.

Pathology and Laboratory Medicine Service, Oklahoma City VA Medical Center, Oklahoma, OK, USA.

出版信息

Methods Mol Biol. 2017;1646:145-151. doi: 10.1007/978-1-4939-7196-1_11.

DOI:10.1007/978-1-4939-7196-1_11

PMID:28804825

Abstract

Protein C (PC) is a plasma Vitamin K-dependent pro-enzyme protein that is synthesized in the liver. Upon activation, PC regulates the coagulation process by neutralizing the procoagulant activities of factors V and VIII in the presence of the cofactor Protein S. PC is a major regulator of the coagulation process. The clotting based Protein C assay, the protocol described in this chapter, quantitates the amount of functional PC present in the specimen in a proportional fashion based on the prolongation of the Activated Partial Thromboplastin Time (APTT). Other methods for assessing PC are also available, including chromogenic and antigenic assays, but protocols for these assays are not provided.

摘要

蛋白C（PC）是一种血浆中依赖维生素K的前酶蛋白，由肝脏合成。激活后，PC在辅因子蛋白S存在的情况下，通过中和因子V和VIII的促凝活性来调节凝血过程。PC是凝血过程的主要调节因子。基于凝血的蛋白C检测（本章所述方法），根据活化部分凝血活酶时间（APTT）的延长，以成比例的方式定量检测样本中功能性PC的含量。评估PC的其他方法也有，包括发色法和抗原法检测，但本章未提供这些检测方法的操作流程。