肝素诱导的血小板减少症（HIT）检测的实验室检测方案。

Laboratory Testing Protocols for Heparin-Induced Thrombocytopenia (HIT) Testing.

作者信息

Lau Kun Kan Edwin, Mohammed Soma, Pasalic Leonardo, Favaloro Emmanuel J

机构信息

Department of Haematology, Institute of Clinical Pathology and Medical Research (ICPMR), NSW Health Pathology, Westmead Hospital, Westmead, NSW, 2145, Australia.

Department of Clinical and Laboratory Haematology, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Westmead, NSW, 2145, Australia.

出版信息

Methods Mol Biol. 2017;1646:227-243. doi: 10.1007/978-1-4939-7196-1_19.

DOI:10.1007/978-1-4939-7196-1_19

PMID:28804833

Abstract

Heparin-induced thrombocytopenia (HIT) represents a significant high morbidity complication of heparin therapy. The clinicopathological diagnosis of HIT remains challenging for many reasons; thus, laboratory testing represents an important component of an accurate diagnosis. Although there are many assays available to assess HIT, these essentially fall into two categories-(a) immunological assays, and (b) functional assays. The current chapter presents protocols for several HIT assays, being those that are most commonly performed in laboratory practice and have the widest geographic distribution. These comprise a manual lateral flow-based system (STiC), a fully automated latex immunoturbidimetric assay, a fully automated chemiluminescent assay (CLIA), light transmission aggregation (LTA), and whole blood aggregation (Multiplate).

摘要

肝素诱导的血小板减少症（HIT）是肝素治疗的一种严重且高发病率的并发症。由于多种原因，HIT的临床病理诊断仍然具有挑战性；因此，实验室检测是准确诊断的重要组成部分。尽管有许多检测方法可用于评估HIT，但这些方法基本上可分为两类——（a）免疫检测法，和（b）功能检测法。本章介绍了几种HIT检测方法的方案，这些方法是实验室实践中最常用且地理分布最广的方法。这些方法包括基于手动侧向流动的系统（STiC）、全自动乳胶免疫比浊法、全自动化学发光检测法（CLIA）、光透射聚集法（LTA）和全血聚集法（Multiplate）。