纤连蛋白与血小板-胶原蛋白黏附的多重相互作用模型
Fibronectin and the multiple interaction model for platelet-collagen adhesion.
作者信息
Santoro S A, Cunningham L W
出版信息
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2644-8. doi: 10.1073/pnas.76.6.2644.
Abstract
A rapid, sensitive, and reproducible assay to determine the adhesion of platelets to collagen has been developed. Collagen fibers and adherent platelets are retained on polycarbonate membrane filters. Chemical modification of collagen by acetylation and of platelets by treatment with chymotrypsin markedly reduces adhesion. The role of fibronectin in the collagen-platelet interaction has been examined. Treatment of platelets with purified antibody or Fab' fragments to fibronectin only slightly reduces adhesion. Preincubation of platelets with high concentrations of gelatin reduces adhesion by only 22% but fails to inhibit aggregation. Thus, fibronectin has only a limited role in the adhesion of platelets to collagen and is either not involved in the adhesion that leads to aggregation or is only one of several adhesion mechanisms, any of which alone can initiate aggregation.
摘要
已开发出一种快速、灵敏且可重复的测定血小板与胶原蛋白黏附的方法。胶原蛋白纤维和黏附的血小板保留在聚碳酸酯膜滤器上。通过乙酰化对胶原蛋白进行化学修饰以及用胰凝乳蛋白酶处理血小板可显著降低黏附。已研究了纤连蛋白在胶原蛋白 - 血小板相互作用中的作用。用纯化的抗纤连蛋白抗体或Fab'片段处理血小板仅轻微降低黏附。用高浓度明胶预孵育血小板仅使黏附降低22%，但未能抑制聚集。因此，纤连蛋白在血小板与胶原蛋白的黏附中仅起有限作用，要么不参与导致聚集的黏附，要么只是几种黏附机制之一，其中任何一种机制单独都可引发聚集。
相似文献
1
Fibronectin and the multiple interaction model for platelet-collagen adhesion.纤连蛋白与血小板-胶原蛋白黏附的多重相互作用模型
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2644-8. doi: 10.1073/pnas.76.6.2644.
2
Platelet-collagen adhesion: evidence for participation of antigenically distinct entities.血小板-胶原蛋白黏附：抗原性不同实体参与的证据。
J Cell Biol. 1984 Dec;99(6):2048-55. doi: 10.1083/jcb.99.6.2048.
3
Platelet-collagen interaction. Inhibition by a monoclonal antibody raised against collagen receptor.血小板与胶原蛋白的相互作用。抗胶原蛋白受体单克隆抗体的抑制作用。
J Immunol. 1987 Aug 1;139(3):887-92.
4
Platelet adhesion.血小板黏附
Prog Hemost Thromb. 1984;7:211-88.
5
Role of factor VIII-von Willebrand factor and fibronectin in the interaction of platelets in flowing blood with monomeric and fibrillar human collagen types I and III.凝血因子VIII-血管性血友病因子和纤连蛋白在流动血液中血小板与单体及纤维状I型和III型人胶原蛋白相互作用中的作用。
J Clin Invest. 1985 Feb;75(2):531-40. doi: 10.1172/JCI111729.
6
Role of thrombospondin in platelet aggregation.血小板反应蛋白在血小板聚集中的作用。
J Clin Invest. 1984 Nov;74(5):1764-72. doi: 10.1172/JCI111595.
7
Fibronectin unfolded by adherent but not suspended platelets: An in vitro explanation for its dual role in haemostasis.纤维连接蛋白在黏附而非悬浮的血小板中展开：其在止血中双重作用的体外解释。
Thromb Res. 2015 Oct;136(4):803-12. doi: 10.1016/j.thromres.2015.08.003. Epub 2015 Aug 5.
8
Preliminary functional characterization of a 24,000 dalton platelet surface protein involved in platelet activation.一种参与血小板激活的24,000道尔顿血小板表面蛋白的初步功能特性分析。
Nouv Rev Fr Hematol (1978). 1985;27(4):255-9.
9
[Adhesion and aggregation of platelets; two states of platelets, stabilization of platelets by serum albumin, and isolation and characterization of plasma proteins to aggregate platelets (author's transl)].[血小板的黏附与聚集；血小板的两种状态、血清白蛋白对血小板的稳定作用以及使血小板聚集的血浆蛋白的分离与特性鉴定（作者译）]
Tanpakushitsu Kakusan Koso. 1979;24(14):1497-507.
10
Interaction of human platelet membrane glycoproteins with collagen and lectins.人血小板膜糖蛋白与胶原蛋白和凝集素的相互作用。
Biochim Biophys Acta. 1984 Jan 24;797(1):10-9.
引用本文的文献
1
Immobilization of Fibronectin-Loaded Polyelectrolyte Nanoparticles on Cardiovascular Material Surface to Improve the Biocompatibility.将负载纤连蛋白的聚电解质纳米粒子固定在心血管材料表面以提高生物相容性。
Biomed Res Int. 2019 Oct 31;2019:5478369. doi: 10.1155/2019/5478369. eCollection 2019.
2
Disorders of platelet function: mechanisms, diagnosis and management.血小板功能障碍：机制、诊断与管理
West J Med. 1981 Feb;134(2):109-27.
3
Role of collagenous matrices in the adhesion and growth of cells.胶原基质在细胞黏附和生长中的作用。
J Cell Biol. 1981 Mar;88(3):473-85. doi: 10.1083/jcb.88.3.473.
4
Platelet stimulation by antifibronectin antibodies requires the Fc region of antibody.抗纤连蛋白抗体对血小板的刺激作用需要抗体的Fc区域。
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6537-40. doi: 10.1073/pnas.79.21.6537.
5
Platelet-collagen adhesion: evidence for participation of antigenically distinct entities.血小板-胶原蛋白黏附：抗原性不同实体参与的证据。
J Cell Biol. 1984 Dec;99(6):2048-55. doi: 10.1083/jcb.99.6.2048.
6
Characterization of human serum spreading factor with monoclonal antibody.用人源单克隆抗体对人血清扩散因子进行特性鉴定。
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1362-6. doi: 10.1073/pnas.80.5.1362.
7
Role of factor VIII-von Willebrand factor and fibronectin in the interaction of platelets in flowing blood with monomeric and fibrillar human collagen types I and III.凝血因子VIII-血管性血友病因子和纤连蛋白在流动血液中血小板与单体及纤维状I型和III型人胶原蛋白相互作用中的作用。
J Clin Invest. 1985 Feb;75(2):531-40. doi: 10.1172/JCI111729.
8
Inhibition of platelet aggregation by a monoclonal antibody against human fibronectin.一种抗人纤连蛋白单克隆抗体对血小板聚集的抑制作用。
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3844-8. doi: 10.1073/pnas.82.11.3844.
9
Exogenous fibronectin modifies the aggregation of collagen-stimulated human platelets.
Experientia. 1990 Jul 15;46(7):680-2. doi: 10.1007/BF01939932.
10
Platelet adhesion to collagen. Factors affecting Mg2(+)-dependent and bivalent-cation-independent adhesion.血小板与胶原蛋白的黏附。影响Mg2(+)-依赖性和二价阳离子非依赖性黏附的因素。
Biochem J. 1990 Jun 1;268(2):481-6. doi: 10.1042/bj2680481.
本文引用的文献
1
One-step growth curve of Western equine encephalomyelitis virus on chicken embryo cells grown in vitro and analysis of virus yields from single cells.西部马脑炎病毒在体外培养的鸡胚细胞上的一步生长曲线及单细胞病毒产量分析
J Exp Med. 1954 Feb;99(2):183-99. doi: 10.1084/jem.99.2.183.
2
Actions of thrombin and other coagulant and proteolytic enzymes on blood platelets.凝血酶及其他凝血和蛋白水解酶对血小板的作用。
Nature. 1967 Dec 2;216(5118):857-8. doi: 10.1038/216857a0.
3
The nature of the intramolecular cross-links in collagen. The separation and characterization of peptides from the cross-link region of rat skin collagen.胶原蛋白分子内交联的性质。大鼠皮肤胶原蛋白交联区域肽段的分离与鉴定。
Biochemistry. 1966 Nov;5(11):3460-73. doi: 10.1021/bi00875a012.
4
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
5
Aggregation of platelets by collagen.血小板与胶原蛋白的聚集。
J Clin Invest. 1968 Dec;47(12):2616-21. doi: 10.1172/JCI105944.
6
Isolation of a collagen-dependent cell attachment factor.一种胶原依赖性细胞黏附因子的分离
Nature. 1974 Jul 19;250(463):248-51. doi: 10.1038/250248a0.
7
Evidence for a structural requirement for the aggregation of platelets by collagen.胶原蛋白诱导血小板聚集的结构要求的证据。
J Clin Invest. 1974 Mar;53(3):875-83. doi: 10.1172/JCI107628.
8
Collagen-mediated platelet aggregation. Effects of collagen modification involving the protein and carbohydrate moieties.胶原蛋白介导的血小板聚集。涉及蛋白质和碳水化合物部分的胶原蛋白修饰的作用。
J Clin Invest. 1973 Oct;52(10):2495-506. doi: 10.1172/JCI107440.
9
Critical role of the carbohydrate side chains of collagen in platelet aggregation.胶原蛋白的碳水化合物侧链在血小板聚集中的关键作用。
J Clin Invest. 1972 Oct;51(10):2693-701. doi: 10.1172/JCI107088.
10
Enzymatic basis for platelet: collagen adhesion as the primary step in haemostasis.血小板的酶学基础：胶原蛋白黏附作为止血的首要步骤。
Nat New Biol. 1971 Nov 3;234(44):5-7. doi: 10.1038/newbio234005a0.
Zotero 插件