Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, China.
CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology , Beijing 100190, China.
Biomacromolecules. 2017 Nov 13;18(11):3506-3513. doi: 10.1021/acs.biomac.7b00780. Epub 2017 Aug 25.
At present, one of main problems for photodynamic therapy (PDT) is how to improve the treatment depth. Two-photon activated (TPA) developed recently provide a possible solution for it. In this work, we report the energy-transferring assembled cationic dipeptide nanoparticles for two-photon activated photodynamic therapy (TPA-PDT). In the nanoparticles, the coencapsulated two-photon fluorescent dye bis(pyrene) (BP) is an energy donor, and a photosensitizer rose bengal (RB) is an acceptor based on an intraparticle fluorescence resonance energy transfer (FRET) mechanism. BP in the nanoparticles can be excited by one- or two- photon laser. And then, the energy of BP was transferred to RB, which highly enhanced the generation of singlet oxygen. The cellular experiments indicated that this nanosystem can induce the cytotoxicity under one- and two-photon irradiation, which allows further applications of FRET-based biomaterials for TPA-PDT.
目前，光动力疗法（PDT）的主要问题之一是如何提高治疗深度。最近开发的双光子激活（TPA）为此提供了一个可能的解决方案。在这项工作中，我们报告了用于双光子激活光动力疗法（TPA-PDT）的能量传递组装阳离子二肽纳米颗粒。在纳米颗粒中，共包封的双光子荧光染料双（芘）（BP）是供体，而基于颗粒内荧光共振能量转移（FRET）机制的光敏剂玫瑰红 B（RB）是受体。纳米颗粒中的 BP 可以被单光子或双光子激光激发。然后，BP 的能量转移到 RB，这大大增强了单线态氧的产生。细胞实验表明，该纳米系统可以在单光子和双光子照射下诱导细胞毒性，这使得基于 FRET 的生物材料在 TPA-PDT 中的进一步应用成为可能。
