Zhao Zhi Jing, Wang Bin, Yuan Jing, Liang Hao Yu, Dong Si Guo, Zeng Ming
National Institutes for Food and Drug Control, Beijing 100050, China.
Institute of Disease Control and Prevention, Academy of Military Medical Sciences, Beijing 100850, China.
Biomed Environ Sci. 2017 Aug;30(8):606-610. doi: 10.3967/bes2017.080.
We used a proteomic approach to identify IbpA in Cronobacter sakazakii (C. sakazaki), which is related to heat tolerance in this strain. The abundance of IbpA in C. sakazakii strains strongly increased after heat shock. C. sakazakii CMCC 45402 ibpA deletion mutants were successfully constructed. The C. sakazakii CMCC 45402 ΔibpA and wild-type strains could not be distinguished based on colony morphology on LB agar plates or biochemical assays. The growth of the C. sakazakii CMCC 45402 ΔibpA mutant in heat shock conditions was indistinguishable from that of the isogenic wild-type, but showed greater heat resistance than E. coli O157:H7 strain CMCC 44828. This study suggests that the absence of a single ibpA gene has no obvious effect on the phenotype or heat resistance of the strain C. sakazakii CMCC 45402.
我们采用蛋白质组学方法在阪崎肠杆菌中鉴定出与该菌株耐热性相关的IbpA。阪崎肠杆菌菌株经热休克后，IbpA的丰度显著增加。成功构建了阪崎肠杆菌CMCC 45402 ibpA缺失突变体。基于LB琼脂平板上的菌落形态或生化分析，无法区分阪崎肠杆菌CMCC 45402 ΔibpA突变体和野生型菌株。阪崎肠杆菌CMCC 45402 ΔibpA突变体在热休克条件下的生长与同基因野生型菌株无异，但比大肠杆菌O157:H7菌株CMCC 44828表现出更强的耐热性。本研究表明，单个ibpA基因的缺失对阪崎肠杆菌CMCC 45402菌株的表型或耐热性没有明显影响。
