酵母质膜H⁺-ATP酶在无质子梯度情况下Pi处于平衡态ATP交换过程中。
Pi in equilibrium ATP exchange in the absence of proton gradient by the H+-ATPase from yeast plasma membranes.
作者信息
de Meis L, Blanpain J P, Goffeau A
出版信息
FEBS Lett. 1987 Feb 23;212(2):323-7. doi: 10.1016/0014-5793(87)81369-8.
Purified soluble H+-ATPase from Schizosaccharomyces pombe catalyzes a Pi in equilibrium ATP exchange in the absence of a H+ gradient. When the pH of the assay medium is raised from 5.5 to 8.0 there is a decrease of the ATPase activity and an increase of the rate of Pi in equilibrium ATP exchange. At pH 7.0 the addition of the organic solvent dimethyl sulfoxide (20%, v/v) promotes a decrease of ATPase activity and an increase of the Pi in equilibrium ATP exchange reaction. The effect of the organic solvent on the Pi in equilibrium ATP exchange is related to a decrease of the apparent Km for Pi.
来自粟酒裂殖酵母的纯化可溶性H⁺-ATP酶在没有H⁺梯度的情况下催化Pi参与的平衡ATP交换反应。当测定介质的pH从5.5升高到8.0时，ATP酶活性降低，而Pi参与的平衡ATP交换速率增加。在pH 7.0时，添加有机溶剂二甲基亚砜（20%，v/v）会导致ATP酶活性降低，同时Pi参与的平衡ATP交换反应速率增加。有机溶剂对Pi参与的平衡ATP交换的影响与Pi的表观Km降低有关。
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