两株炭疽芽孢杆菌的基因组测序：一株强毒株和一株疫苗株。

Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strain.

作者信息

Siqueira Franciele Maboni, Cibulski Samuel Paulo, Mayer Fabiana Quoos, Driemeier David, Pavarini Saulo Petinatti, Vargas Agueda Palmira Castagna de

机构信息

Universidade Federal do Rio Grande do Sul (UFRGS), Faculdade de Veterinária, Departamento de Patologia Clínica Veterinária, Porto Alegre, RS, Brazil.

出版信息

Braz J Microbiol. 2018 Jan-Mar;49(1):18-19. doi: 10.1016/j.bjm.2017.04.007. Epub 2017 Jul 31.

DOI:10.1016/j.bjm.2017.04.007

PMID:28807610

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5790590/

Abstract

Bacillus anthracis strain SPV842_15 was isolated from bovine fetus, while B. anthracis strain Brazilian vaccinal was recovered from a commercial vaccine. We report here the genome sequences of both strains. The SPV842_15 genome is composed of a single circular chromosome with a length of 5,228,664 base pairs, and comprises 5911 coding sequences. In turn, the Brazilian vaccinal genome remains in 201 contigs with 5733 coding sequences. Both genomes have an overall C+G content of 35.4%, and 11 genes encoding the ribosomal RNAs (rRNAs) 5S, 16S and 23S. Only the plasmid pX01 sequence, which carries genes for toxins synthesis, was detected and completely assembled for both strains. These plasmids have a length of 181,684 base pairs and a C+G content of 32.5%. These genomic data generate insights about vaccinal B. anthracis virulence.

摘要

炭疽芽孢杆菌菌株SPV842_15是从牛胎儿中分离出来的，而炭疽芽孢杆菌巴西疫苗株是从一种商业疫苗中获得的。我们在此报告这两种菌株的基因组序列。SPV842_15基因组由一条长度为5,228,664个碱基对的单一环状染色体组成，包含5911个编码序列。相比之下，巴西疫苗株基因组由201个重叠群组成，有5733个编码序列。两种基因组的总体C+G含量均为35.4%，有11个基因编码5S、16S和23S核糖体RNA（rRNA）。仅检测到并完整组装了两种菌株携带毒素合成基因的质粒pX01序列。这些质粒长度为181,684个碱基对，C+G含量为32.5%。这些基因组数据为炭疽芽孢杆菌疫苗株的毒力提供了见解。