Napavichayanun Supamas, Lutz Oliver, Fischnaller Martin, Jakschitz Thomas, Bonn Günther, Aramwit Pornanong
Bioactive Resources for Innovative Clinical Applications Research Unit, Chulalongkorn University, Phatumwan, Bangkok 10330, Thailand; Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, PhayaThai Road, Phatumwan, Bangkok 10330, Thailand.
Austrian Drug Screening Institute (ADSI), University of Innsbruck, Innrain 66a, Innsbruck, 6020, Austria.
Arch Biochem Biophys. 2017 Oct 1;631:58-65. doi: 10.1016/j.abb.2017.08.010. Epub 2017 Aug 12.
Silk cocoon is produced from silkworm (Bombyx mori) to protect itself from outer environment. Various strains of cocoon contain different forms and amounts of flavonoids, which may affect on their antioxidant activity. Moreover, the extraction method would influence the amount of flavonoids extracted. Therefore, the objectives of this study were to identify and quantify the flavonoids in 3 strains of bivoltine Bombyx mori silk cocoon (Chul 1/1; white cocoon, Chul 3/2; greenish cocoon, and Chul 4/2; yellow cocoon) extracted by 6 different solvents including acetone, ethyl acetate, dimethyl sulfoxide (DMSO), ethanol, methanol, and purified water. The flavonoids extracted were identified and quantified by liquid chromatography-mass spectrometry (LC-MS). The antioxidant activity of flavonoids extracted was also investigated by visible spectroscopy at 517 nm. The results showed that Chul 3/2 silk cocoon contained the highest amount of flavonoids. Purified water seemed to be the best solvent that preserved most antioxidant activity of the flavonoids extracted. Flavonoids in Chul 1/1 and Chul 4/2 silk cocoon were rarely found, however they contained some antioxidant activities. The data from this study can provide basic information for flavonoid extraction from silk cocoon which can also apply for other flavonoid-containing natural biomaterials.
蚕茧由蚕（家蚕）吐出，用于保护自身免受外部环境影响。不同品系的蚕茧含有不同形式和数量的黄酮类化合物，这可能会影响它们的抗氧化活性。此外，提取方法会影响黄酮类化合物的提取量。因此，本研究的目的是鉴定和定量3个二化性家蚕品系（Chul 1/1；白茧、Chul 3/2；绿茧和Chul 4/2；黄茧）中用6种不同溶剂（包括丙酮、乙酸乙酯、二甲基亚砜（DMSO）、乙醇、甲醇和纯净水）提取的黄酮类化合物。通过液相色谱 - 质谱联用（LC - MS）对提取的黄酮类化合物进行鉴定和定量。还通过在517 nm处的可见光谱研究了提取的黄酮类化合物的抗氧化活性。结果表明，Chul 3/2蚕茧中黄酮类化合物含量最高。纯净水似乎是保留提取的黄酮类化合物大部分抗氧化活性的最佳溶剂。在Chul 1/1和Chul 4/2蚕茧中很少发现黄酮类化合物，然而它们含有一些抗氧化活性。本研究的数据可为从蚕茧中提取黄酮类化合物提供基础信息，这也可应用于其他含黄酮类化合物的天然生物材料。
