Wang Wei-Hsun, Lin Wen-Shin, Shih Chia-Hung, Chen Cheng-You, Kuo Siao-Hong, Li Wei-Lin, Lin Yung-Sheng
Department of Orthopaedics, Changhua Christian Hospital, Changhua 500209, Taiwan.
School of Medicine, Kaohsiung Medical University, Kaohsiung 807378, Taiwan.
Materials (Basel). 2021 Sep 15;14(18):5314. doi: 10.3390/ma14185314.
Sericin, a textile waste, can be used for antioxidant and skin-whitening purposes. The hydrothermal method of extracting sericin is more eco-friendly than are chemical and enzymatic methods. In this study, silk cocoons were cut into pieces and then subjected to hydrothermal extraction at three temperatures (160, 200, and 220 °C) to obtain sericin extracts (Sericin160, Sericin200, and Sericin220, respectively). Antioxidant activity and tyrosinase inhibition were measured to determine the extracts' effectiveness. Sericin220 was the strongest antioxidant, with total phenol content, total flavonoid content, and ferric reducing power of 62.19 ± 0.04 mg gallic acid equivalents/g dry weight, 0.07 ± 0.01 mg quercetin equivalent/g dry weight, and 181.49 ± 0.024 mg vitamin C equivalent/g dry weight, respectively. The half-maximal inhibitory concentrations for DPPH and ABTS free-radical scavenging ability were 6.41 ± 0.05 and 0.79 ± 0.37 mg/mL, respectively. Sericin220 also exhibited the highest tyrosinase inhibition activity (70.82 ± 4.1 mg vitamin C equivalent/g), indicating its whitening potential.
丝胶蛋白是一种纺织废料，可用于抗氧化和美白皮肤。与化学法和酶法相比，水热法提取丝胶蛋白更环保。在本研究中，将蚕茧切成小块，然后在三个温度（160、200和220°C）下进行水热提取，以获得丝胶提取物（分别为Sericin160、Sericin200和Sericin220）。通过测量抗氧化活性和酪氨酸酶抑制作用来确定提取物的有效性。Sericin220是最强的抗氧化剂，其总酚含量、总黄酮含量和铁还原能力分别为62.19±0.04毫克没食子酸当量/克干重、0.07±0.01毫克槲皮素当量/克干重和181.49±0.024毫克维生素C当量/克干重。DPPH和ABTS自由基清除能力的半数抑制浓度分别为6.41±0.05和0.79±0.37毫克/毫升。Sericin220还表现出最高的酪氨酸酶抑制活性（70.82±4.1毫克维生素C当量/克），表明其具有美白潜力。
