Department of Pharmaceutical Botany, Faculty of Pharmacy, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Department of Pharmacy, "G. d'Annunzio" University of Chieti - Pescara, Via dei Vestini 31, 66100 Chieti, Italy.
Food Chem Toxicol. 2018 Feb;112:441-447. doi: 10.1016/j.fct.2017.08.009. Epub 2017 Aug 12.
Kudzu, the dried root of an important edible plant (Pueraria lobata), is used in Traditional Chinese Medicine for the important nutritional value strictly related to its isoflavone derivatives. These compounds characterize the quality of kudzu contained in different preparations, as pharmaceutical ingredient as well as dietary/food supplement (e.g. starch). The optimization of the isoflavones recovery, monitored by HPLC-PDA, through different innovative and conventional extraction techniques, e.g. microwave-assisted, ultrasound-assisted and conventional extraction, represented a suitable challenge in food industry and natural products evaluation. The impact on the isoflavone extraction by using an ionic liquid-assisted procedure was also considered. Furthermore, the inhibitory activity of the most representative isoflavones, isolated from kudzu, was evaluated using four isoforms (I, II, IX and XII) of human carbonic anhydrase (hCA) due to their role in several physiopathological processes.
葛，一种重要食用植物（野葛）的干燥根，在中国传统医学中被用于其异黄酮衍生物所具有的重要营养价值。这些化合物是不同制剂中葛的质量特征，既是药物成分，也是膳食/食品补充剂（如淀粉）。通过高效液相色谱-光电二极管阵列（HPLC-PDA）监测，通过不同的创新和常规提取技术（如微波辅助、超声辅助和常规提取）优化异黄酮的回收，这在食品工业和天然产物评价中是一个合适的挑战。还考虑了离子液体辅助法对异黄酮提取的影响。此外，还评估了从葛中分离出的最具代表性的异黄酮对四种人碳酸酐酶（hCA）同工型（I、II、IX 和 XII）的抑制活性，因为它们在多种生理病理过程中发挥作用。
