CRP 异构体与氧化型低密度脂蛋白（oxLDL）的组合可减少 U937 来源的巨噬细胞释放肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）。
The combination of CRP isoforms with oxLDL decreases TNF-α and IL-6 release by U937-derived macrophages.
作者信息
Krayem Imtissal, Bazzi Samer, Karam Marc
机构信息
Department of Biology, Faculty of Sciences, University of Balamand, Deir El Balamand, El-Koura, 100-Tripoli, Lebanon.
出版信息
Biomed Rep. 2017 Sep;7(3):272-276. doi: 10.3892/br.2017.949. Epub 2017 Jul 21.
Abstract
C-reactive protein (CRP) and oxidized low density lipoprotein (oxLDL) serve major roles at both early and advanced stages of atherosclerosis. CRP exists in two isoforms, monomeric (m) and pentameric (p), that bring about pro- or anti-inflammatory effects in macrophages. In addition, CRP may form a complex with oxidized low-density lipoprotein (oxLDL) via phosphatidylcholine, thus decreasing its pro-inflammatory effects within macrophages. The aim of the present study was to investigate the single and the combined effects of mCRP, pCRP and oxLDL on U937-derived macrophages. In the current study, U937-derived macrophages were treated with different combinations of CRP isoforms with or without oxLDL. The levels of major inflammatory cytokines [interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor (TNF)-α] along with the production of reactive oxygen species (ROS) were determined. TNF-α and IL-6 levels were significantly decreased (P<0.05) by the effect of mCRP and pCRP combined with oxLDL. No significant changes were observed in IL-1β, IL-8 or ROS levels.
摘要
C反应蛋白（CRP）和氧化型低密度脂蛋白（oxLDL）在动脉粥样硬化的早期和晚期均发挥主要作用。CRP存在两种异构体，即单体（m）和五聚体（p），它们在巨噬细胞中产生促炎或抗炎作用。此外，CRP可通过磷脂酰胆碱与氧化型低密度脂蛋白（oxLDL）形成复合物，从而降低其在巨噬细胞内的促炎作用。本研究的目的是探讨mCRP、pCRP和oxLDL对U937衍生巨噬细胞的单一及联合作用。在本研究中，用含或不含oxLDL的不同CRP异构体组合处理U937衍生巨噬细胞。测定主要炎性细胞因子[白细胞介素（IL）-1β、IL-6、IL-8和肿瘤坏死因子（TNF）-α]的水平以及活性氧（ROS）的产生。mCRP和pCRP与oxLDL联合作用使TNF-α和IL-6水平显著降低（P<0.05）。IL-1β、IL-8或ROS水平未观察到显著变化。
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