Dupnock Trisha L, Deshusses Marc A
Department of Civil and Environmental Engineering, Duke University, 127C Hudson Hall; Box 90287, Durham, NC, 27708-0287, USA.
Appl Biochem Biotechnol. 2017 Oct;183(2):488-502. doi: 10.1007/s12010-017-2569-2. Epub 2017 Aug 14.
This research reports the development of a biotrickling filter (BTF) to upgrade biogas, which is achieved by adding H to reduce CO. H and CO (80:20% vol.) were fed to a bench-scale BTF packed with polyurethane foam (PUF) and inoculated with hydrogenotrophic methanogens. Maximum CH production rates recorded were as high as 38 m m day, which is 5-30 times faster than earlier reports with other kinds of bioreactors. The high rates were attributed to the efficient mass transfer and high density of methanogens in the BTF. The removal efficiencies for H and CO were 83 and 96%, respectively. 5-Cyano-2,3-ditolyl tetrazolium chloride/DAPI staining revealed that 67% of cells were alive near the gas entrance port, while only 8.3% were alive at the exit. Furthermore, DNA sequencing showed that only 27% of the biomass was composed of Euryarchaeota, the phylum which includes methanogens. These two observations suggest that optimizing the methanogen density and activity could possibly reach even higher biogas upgrading rates.
本研究报告了一种用于提升沼气品质的生物滴滤池（BTF）的开发情况，该过程通过添加氢气来还原二氧化碳得以实现。将氢气和二氧化碳（体积比80:20%）输送至一个装有聚氨酯泡沫（PUF）并接种了嗜氢产甲烷菌的实验室规模生物滴滤池中。记录到的最大甲烷产率高达38立方米/天，这比之前其他类型生物反应器的报道快5至30倍。高反应速率归因于生物滴滤池中高效的传质过程和高密度的产甲烷菌。氢气和二氧化碳的去除效率分别为83%和96%。5-氰基-2,3-二苯基四氮唑氯化物/4',6-二脒基-2-苯基吲哚染色显示，在气体入口附近67%的细胞存活，而在出口处仅有8.3%的细胞存活。此外，DNA测序表明，仅有27%的生物质由广古菌门组成，该门包括产甲烷菌。这两个观察结果表明，优化产甲烷菌的密度和活性可能会实现更高的沼气提升速率。
