大鼠肝脏去细胞化与器官发生

Decellularization of Liver and Organogenesis in Rats.

作者信息

Shirakigawa Nana, Ijima Hiroyuki

机构信息

Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Fukuoka, Japan.

出版信息

Methods Mol Biol. 2018;1577:271-281. doi: 10.1007/7651_2017_63.

DOI:10.1007/7651_2017_63

PMID:28808979

Abstract

Recently, organ construction has been attempted using decellularized organs. In this study, we used decellularized rat liver to construct liver tissue by recellularization. The right lobe of the rat liver was decellularized with 4% Triton X-100 solution, recellularized with 10 rat hepatocytes, and albumin synthesis in the recellularized right lobe was observed. Therefore, we introduce a method of decellularizing rat liver, which retains its fine vascular structure after removal of all the cells, perform organogenesis using the decellularized liver, and evaluate the structural and functional properties of the products.

摘要

最近，人们尝试使用去细胞化器官构建组织器官。在本研究中，我们使用去细胞化大鼠肝脏通过再细胞化构建肝组织。用4% Triton X - 100溶液对大鼠肝脏右叶进行去细胞化处理，再用10个大鼠肝细胞进行再细胞化，并观察再细胞化右叶中白蛋白的合成情况。因此，我们介绍一种对大鼠肝脏进行去细胞化处理的方法，该方法在去除所有细胞后仍保留其精细的血管结构，利用去细胞化肝脏进行器官生成，并评估产物的结构和功能特性。