Uchikawa Masanori, Nishimura Naoko, Iwafuchi-Doi Makiko, Kondoh Hisato
Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Shizuoka Prefecture Special Education Schools, Yahatano, Ito, Shizuoka, 413-0232, Japan.
Methods Mol Biol. 2017;1650:191-202. doi: 10.1007/978-1-4939-7216-6_12.
Chicken embryo electroporation is a powerful tool used to identify and analyze enhancers involved in developmental gene regulation. In this chapter, the basic procedures and underlying principles of enhancer analysis using chicken embryo electroporation are described in the following steps: (1) identification of enhancers in a wide genomic region, (2) determination of the full enhancer region, (3) definition of the core enhancer regions, and (4) analysis of a functional transcription factor binding sequences in the core region.
鸡胚电穿孔是一种用于鉴定和分析参与发育基因调控的增强子的强大工具。在本章中，使用鸡胚电穿孔进行增强子分析的基本程序和基本原理按以下步骤描述：（1）在广泛的基因组区域中鉴定增强子，（2）确定完整的增强子区域，（3）定义核心增强子区域，以及（4）分析核心区域中的功能性转录因子结合序列。
