IEEE Trans Biomed Circuits Syst. 2017 Dec;11(6):1450-1458. doi: 10.1109/TBCAS.2017.2729584. Epub 2017 Aug 14.
The distinct motion of GFP-tagged histone expressing cells (Histone-GFP type cells) has been investigated under ac electrokinetics in an electrode-multilayered microfluidic device as compared with Wild type cells and GFP type cells in terms of different intracellular components. The Histone-GFP type cells were modified by the transfection of green fluorescent protein-fused histone from the human lung fibroblast cell line. The velocity of the Histone-GFP type cells obtained by particle tracking velocimetry technique is faster than Wild type cells by 24.9% and GFP type cells by 57.1%. This phenomenon is caused by the more amount of proteins in the intracellular of single Histone-GFP type cell than that of the Wild type and GFP type cells. The more amount of proteins in the Histone-GFP type cells corresponds to a lower electric permittivity ϵ of the cells, which generates a lower dielectrophoretic force exerting on the cells. The velocity of Histone-GFP type cells is well agreed with Eulerian-Lagrangian two-phase flow simulation by 4.2% mean error, which proves that the fluid motion driven by thermal buoyancy and electrothermal force dominates the direction of cells motion, while the distinct motion of Histone-GFP type cells is caused by dielectrophoretic force. The fluid motion does not generate a distinct drag motion for Histone-GFP type cells because the Histone-GFP type cells have the same size to the Wild type and GFP type cells. These results clarified the mechanism of cells motion in terms of intracellular components, which helps to improve the cell manipulation efficiency with electrokinetics.
在电极多层微流控装置中，通过对比 GFP 标记组蛋白表达细胞（Histone-GFP 型细胞）与野生型细胞和 GFP 型细胞的不同细胞内成分，研究了在交流电动力学作用下 GFP 标记组蛋白表达细胞（Histone-GFP 型细胞）的独特运动。Histone-GFP 型细胞通过转染来自人肺成纤维细胞系的绿色荧光蛋白融合组蛋白进行修饰。通过粒子追踪速度测量技术获得的 Histone-GFP 型细胞的速度比野生型细胞快 24.9%，比 GFP 型细胞快 57.1%。这种现象是由于单个 Histone-GFP 型细胞的细胞内蛋白质含量比野生型和 GFP 型细胞多。Histone-GFP 型细胞内更多的蛋白质对应于细胞的介电常数 ε 更低，这会产生对细胞施加的更低的介电泳力。Histone-GFP 型细胞的速度与 Eulerian-Lagrangian 两相流模拟吻合良好，平均误差为 4.2%，这证明了由热浮力和电热力驱动的流体运动主导了细胞运动的方向，而 Histone-GFP 型细胞的独特运动是由介电泳力引起的。由于 Histone-GFP 型细胞与野生型和 GFP 型细胞具有相同的大小，因此流体运动不会对 Histone-GFP 型细胞产生明显的阻力运动。这些结果阐明了细胞运动的机制，根据细胞内成分，可以帮助提高电动力学的细胞操作效率。
