Centro de Investigación Interdisciplinario, Área Académica de Nutrición, Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Circuito Actopan-Tilcuautla s/n, Ex hacienda La Concepción, San Agustín Tlaxiaca, Hidalgo 42160, Mexico.
Área Académica de Química, Universidad Autónoma del Estado de Hidalgo, Km 4.5 Carretera Pachuca-Tulancingo, Mineral de la Reforma, Hidalgo 42184, Mexico.
Molecules. 2017 Aug 15;22(8):1344. doi: 10.3390/molecules22081344.
The aim of this study was to evaluate the stability of color, betaxanthin, and betacyanin pigments in the presence of Cu(II)-dependent hydroxyl radicals (HO•) from ultrasonicated purple cactus pear juice at amplitudes of 40%, 60%, and 80%, in comparison to untreated sample. L* parameter of juice treated at 40% and 80% amplitude for 25 and 15 min, respectively (11.3 and 9.3, respectively), were significantly higher compared to the control; b* and hue parameters of juice treated at 80%, 25 min showed values of 1.7 and 0.1, respectively. Color differences (Δ) were lower (<3) for juices treated at high amplitude (80%) and short times (3-5 min). Juice treated at 40% 15 min, 60% 25 min, 80% 15 and 25 min presented high values of betacyanins (281.7 mg·L, 255.9 mg·L, 294.4 mg·L, and 276.7 mg·L, respectively). Betaxanthin values were higher in the juices treated at 40% 5 min and 80% 15 and 25 min (154.2 mg·L, 135.2 mg·L, and 128.5 mg·L, respectively). Purple cactus pear juice exhibited significant chelating activity of copper ions and great stability when exposed to HO•.
本研究旨在评估超声处理的紫色仙人掌梨汁在 Cu(II)依赖性羟基自由基 (HO•) 存在下的颜色、甜菜黄素和甜菜红素稳定性,与未处理的样品相比。在 40%和 80%的振幅下分别处理 25 和 15 分钟的果汁的 L参数(分别为 11.3 和 9.3)显著高于对照;在 80%振幅下处理的 b和色调参数果汁,25 分钟时的数值分别为 1.7 和 0.1。在高振幅(80%)和短时间(3-5 分钟)处理下,果汁的颜色差异(Δ)较低(<3)。在 40%处理 15 分钟、60%处理 25 分钟、80%处理 15 分钟和 25 分钟的果汁中,甜菜红素含量较高(分别为 281.7 mg·L、255.9 mg·L、294.4 mg·L 和 276.7 mg·L)。在 40%处理 5 分钟和 80%处理 15 分钟和 25 分钟的果汁中,甜菜黄素值较高(分别为 154.2 mg·L、135.2 mg·L 和 128.5 mg·L)。紫色仙人掌梨汁在暴露于 HO•时表现出显著的铜离子螯合活性和良好的稳定性。