Li Xiaobo, Ma Min, Zhang Xianggui, Deng Liang, Wang Yarong, Bian Zhuang, Cai Shining, Peng Bangya, Yang Jiangquan, Chen Yang
College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong 510632, P.R. China.
Department of Diagnostics, Zhuhai Campus of Zunyi Medical University, Zhuhai, Guangdong 519041, P.R. China.
Exp Ther Med. 2017 Aug;14(2):1381-1388. doi: 10.3892/etm.2017.4662. Epub 2017 Jun 23.
Gardenia fruit has been used in traditional Chinese medicine for thousands of years. A previous study by the present authors indicated that the ethanol extract of gardenia fruits (EEG) primarily contains eight constituents. In the present study, the potential effects of EEG on unilateral ureteral obstruction (UUO)-induced renal interstitial fibrosis were observed in rats. A total of 30 rats were randomly divided into three groups (n=10 each): Sham group, UUO group, and EEG group, which were administered with EEG (200 mg/kg/day) or the same volume of distilled water as a vehicle. UUO were established by ligating left ureter at two points and cut between the ligatures. All rats were sacrificed at 14 days after UUO operation. the present results demonstrated that EEG significantly elevated the expressions of vascular endothelial growth factor and E-cadherin induced by UUO (both P<0.05), and reduced levels of hypoxia-inducible factor-1α, transforming growth factor-β1, connective tissue growth factor and α-smooth muscle actin (all P<0.05). The present findings suggest that EEG is a potential novel renoprotective compound for renal fibrosis through inhibiting epithelial-to-mesenchymal transition.
栀子果实应用于传统中药已有数千年历史。本研究作者之前的一项研究表明，栀子果实乙醇提取物（EEG）主要含有八种成分。在本研究中，观察了EEG对单侧输尿管梗阻（UUO）诱导的大鼠肾间质纤维化的潜在影响。总共30只大鼠随机分为三组（每组n = 10）：假手术组、UUO组和EEG组，EEG组给予EEG（200 mg/kg/天）或相同体积的蒸馏水作为溶媒。通过在两点结扎左输尿管并在结扎之间切断来建立UUO。所有大鼠在UUO手术后14天处死。目前的结果表明，EEG显著提高了UUO诱导的血管内皮生长因子和E-钙黏蛋白的表达（均P<0.05），并降低了缺氧诱导因子-1α、转化生长因子-β1、结缔组织生长因子和α-平滑肌肌动蛋白的水平（均P<0.05）。目前的研究结果表明，EEG可能是一种通过抑制上皮-间充质转化对肾纤维化具有潜在保护作用的新型化合物。
